PXD001051

PXD001051 is an original dataset announced via ProteomeXchange.

Title	Shotgun proteome analysis of the bacterium Clostridium cellulolyticum
Description	Biofuel production from lignocellulosic waste and residues is a promising option to mitigate the environmental costs associated to energy production. However, the difficulty to cost-effectively overcome lignocellulose recalcitrance hampers a widespread application of such bioprocesses. Through an integrated approach, we focused on the factors affecting cellulose reactivity and their impact on downstream fermentation. Three cellulosic manufactured materials were characterized in details: facial tissue, Whatman paper, cotton pads. The model mesophilic cellulolytic bacterium Clostridium cellulolyticum was used to study colonization and metabolic patterns during fermentation of these materials. Facial tissue was extensively colonized and exhibited the fastest degradation and the highest ethanol-to-acetate ratio. Comparing facial tissue fermentation to Whatman paper fermentation by label-free quantitative shotgun proteomics and statistical analyses, 187 proteins showed a different behavior; higher concentration levels were detected for many enzymes from the carbohydrate central metabolic pathway; distinct patterns of expression levels were observed for carbohydratases degrading cellulose and hemicellulose. Overall, lower degrees of polymerization, lower crystallinity index, and the presence of hemicelluloses could explain the higher biological reactivity and bioethanol production yields.
HostingRepository	PRIDE
AnnounceDate	2017-01-30
AnnouncementXML	Submission_2017-01-30_12:51:05.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001051
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ariane Bize
SpeciesList	scientific name: Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10); NCBI TaxID: 394503;
ModificationList	dehydrated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; deaminated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Discovery

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-11 01:05:55	ID requested
⏵ 1	2017-01-30 12:51:06	announced

Badalato N, Guillot A, Sabarly V, Dubois M, Pourette N, Pontoire B, Robert P, Bridier A, Monnet V, Sousa DZ, Durand S, Maz, é, as L, Bul, é, on A, Bouchez T, Mortha G, Bize A, Whole Proteome Analyses on Ruminiclostridium cellulolyticum Show a Modulation of the Cellulolysis Machinery in Response to Cellulosic Materials with Subtle Differences in Chemical and Structural Properties. PLoS One, 12(1):e0170524(2017) [pubmed]

submitter keyword: Clostridium cellulolyticum, label-free quantitative proteomics, cellulose, bioethanol, bioenergy, LC-MS/MS

Théodore Bouchez
contact affiliation	Biomic Team, Environmental Technologies Department, Irstea, France
contact email	theodore.bouchez@irstea.fr
lab head
Ariane Bize
contact affiliation	Irstea
contact email	ariane.bize@irstea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001051
     1. Label: PRIDE project
     2. Name: Shotgun proteome analysis of the bacterium Clostridium cellulolyticum