PXD001046

PXD001046 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics analysis of superior and inferior spikelets in hybrid rice during grain filling and response of inferior spikelets to drought stress using isobaric tags for relative and absolute quantification
Description	The biological functions of differently expressed proteins between superior and inferior spikelet grains were investigated based on the isobaric tags for relative and absolute quantification to further clarify the mechanism of rice grain filling at the proteomic level, as well as the response of inferior spikelets to drought dress (-20 kPa or -40 kPa). Compared with superior spikelets, inferior ones had lower sink strength due to the lower sink activities (lower expressions of ADP-glucose pyrophosphorylase, granule-bound starch synthase, starch branching enzyme and pullulanase) and smaller sink sizes (lower abundances of structural proteins). The slower and later grain filling resulted from the weaker decomposition and conversion of photoassimilate and the slower cell division. Moderate drought stress (-20 kPa) promoted the grain filling of inferior spikelets through regulating the proteins associated with photoassimilate supply and conversion. These proteins may be important targets for rice breeding programs that raise the rice yield under drought condition. The findings offer new insights into rice grain-filling and provide theoretical evidences for better quality control and scientific improvement of super rice in practice.
HostingRepository	PRIDE
AnnounceDate	2015-08-05
AnnouncementXML	Submission_2015-08-05_04:39:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Minghui Dong
SpeciesList	scientific name: Rice(O. sativa); NCBI TaxID: 4530;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-09 02:06:48	ID requested
⏵ 1	2015-08-05 04:39:47	announced

Dong M, Gu J, Zhang L, Chen P, Liu T, Deng J, Lu H, Han L, Zhao B, Data in support of comparative proteomics analysis of superior and inferior spikelets in hybrid rice during grain filling and response of inferior spikelets to drought stress using isobaric tags for relative and absolute quantification. Data Brief, 1():51-5(2014) [pubmed]

curator keyword: Biological

submitter keyword: hybrid rice

grain filling

drought stress

iTRAQ

proteome

Minghui Dong
contact affiliation	suzhou academy of agricultural sciences
contact email	306860631@qq.com
lab head
Minghui Dong
contact affiliation	SUZHOU ACADEMY OF SCIENCE
contact email	306860631@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001046
     1. Label: PRIDE project
     2. Name: Comparative proteomics analysis of superior and inferior spikelets in hybrid rice during grain filling and response of inferior spikelets to drought stress using isobaric tags for relative and absolute quantification