PXD001041 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Divergent Cysteines in FOXO3 and FOXO4 Mediate Paralog-Specific Redox Signaling |
Description | Reduction-oxidation (redox) signaling, the translation of an oxidative intracellular environment into a cellular response, is mediated by the reversible oxidation of specific cysteine thiols. The latter results in disulfide formation between protein (hetero)dimers that alter protein function until the cellular redox has returned to the basal state. We have previously shown that this mechanism promotes the nuclear localization and activity of the FOXO4 transcription factor. Here, we present evidence that FOXO3 and FOXO4 have acquired paralog-specific cysteines throughout vertebrate evolution. Using a proteome-wide screen we identified previously unknown redox-dependent FOXO3 interaction partners. The nuclear import receptor IPO7 forms a disulfide-dependent heterodimer with FOXO3, but not with FOXO4, which is required for reactive oxygen species (ROS)-induced nuclear translocation . These findings suggest that evolutionary acquisition of cysteines has contributed to functional divergence of FOXO paralogs. |
HostingRepository | PRIDE |
AnnounceDate | 2014-10-23 |
AnnouncementXML | Submission_2014-10-23_03:57:56.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Harmjan Vos |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | N-ethylmaleimide derivatized cysteine; deamidated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-06-06 03:58:18 | ID requested | |
1 | 2014-08-26 06:50:25 | announced | |
⏵ 2 | 2014-10-23 03:58:01 | announced | Updated project metadata. |
Publication List
Putker M, Vos HR, van Dorenmalen K, de Ruiter H, Duran AG, Snel B, Burgering BM, Vermeulen M, Dansen TB, Evolutionary acquisition of cysteines determines FOXO paralog-specific redox signaling. Antioxid Redox Signal, 22(1):15-28(2015) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: foxo3 Reduction-oxidation (redox) signaling |
Contact List
Tobias Dansen |
contact affiliation | Tobias B. Dansen, Ph.D. University Medical Center Utrecht Dept. Molecular Cancer Research, Universiteitsweg 100, STR 3.203 3584 CG Utrecht, The Netherlands |
contact email | T.B.Dansen@umcutrecht.nl |
lab head | |
Harmjan Vos |
contact affiliation | University Medical Center Utrecht Dept. Molecular Cancer Research |
contact email | h.r.vos-3@umcutrecht.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001041
- Label: PRIDE project
- Name: Divergent Cysteines in FOXO3 and FOXO4 Mediate Paralog-Specific Redox Signaling