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PXD001041

PXD001041 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDivergent Cysteines in FOXO3 and FOXO4 Mediate Paralog-Specific Redox Signaling
DescriptionReduction-oxidation (redox) signaling, the translation of an oxidative intracellular environment into a cellular response, is mediated by the reversible oxidation of specific cysteine thiols. The latter results in disulfide formation between protein (hetero)dimers that alter protein function until the cellular redox has returned to the basal state. We have previously shown that this mechanism promotes the nuclear localization and activity of the FOXO4 transcription factor. Here, we present evidence that FOXO3 and FOXO4 have acquired paralog-specific cysteines throughout vertebrate evolution. Using a proteome-wide screen we identified previously unknown redox-dependent FOXO3 interaction partners. The nuclear import receptor IPO7 forms a disulfide-dependent heterodimer with FOXO3, but not with FOXO4, which is required for reactive oxygen species (ROS)-induced nuclear translocation . These findings suggest that evolutionary acquisition of cysteines has contributed to functional divergence of FOXO paralogs.
HostingRepositoryPRIDE
AnnounceDate2014-10-23
AnnouncementXMLSubmission_2014-10-23_03:57:56.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHarmjan Vos
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListN-ethylmaleimide derivatized cysteine; deamidated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-06-06 03:58:18ID requested
12014-08-26 06:50:25announced
22014-10-23 03:58:01announcedUpdated project metadata.
Publication List
Putker M, Vos HR, van Dorenmalen K, de Ruiter H, Duran AG, Snel B, Burgering BM, Vermeulen M, Dansen TB, Evolutionary acquisition of cysteines determines FOXO paralog-specific redox signaling. Antioxid Redox Signal, 22(1):15-28(2015) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: foxo3 Reduction-oxidation (redox) signaling
Contact List
Tobias Dansen
contact affiliationTobias B. Dansen, Ph.D. University Medical Center Utrecht Dept. Molecular Cancer Research, Universiteitsweg 100, STR 3.203 3584 CG Utrecht, The Netherlands
contact emailT.B.Dansen@umcutrecht.nl
lab head
Harmjan Vos
contact affiliationUniversity Medical Center Utrecht Dept. Molecular Cancer Research
contact emailh.r.vos-3@umcutrecht.nl
dataset submitter
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Dataset FTP location
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