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PXD001039

PXD001039 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphoproteomics of the Milk Fat Globule Membrane: new insights into lipid droplet secretion from the mammary epithelial cell
DescriptionLipid droplets are secreted into milk by a complex process which begins by burgeoning oflipids at the endoplasmic reticulum of the mammary epithelial cell (MEC). Lipid dropletsenveloped by organelle-derived phospholipids reach the apical pole of the cell where they arewrapped by the plasma membrane to be secreted as fat globules into milk. Analyzing the fineprotein composition of the Milk Fat Globule Membrane (MFGM), the triple-layeredmembrane surrounding milk lipid droplets can therefore provide mechanistic clues to betterunderstand lipid droplet biosynthesis and secretion pathways. We combined a high sensitiveQ Exactive LC-MS/MS analysis of MFGM-derived peptides with the use of an in-house database intended to improve protein identification in the goat species. Using this approach, we performed the identification of 442 functional groups of proteins in the MFGM from goatmilk. To get a more comprehensive view of intracellular mechanisms driving lipid dropletdynamics in the MEC, we decided to investigate for the first time whether MFGM proteinswere phosphorylated. A phosphopeptide enrichment approach let us pinpoint 271 sites ofphosphorylation on 124 unique goat MFGM proteins. Enriched gene ontology termsassociated with phosphorylated MFGM proteins were protein transport and actin cytoskeletonorganization. Gained data are discussed with regard to lipid secretory mechanisms in the MEC.
HostingRepositoryPRIDE
AnnounceDate2015-05-12
AnnouncementXMLSubmission_2015-05-12_02:12:09.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHENRY CELINE
SpeciesList scientific name: Capra hircus (Goat); NCBI TaxID: 9925;
ModificationListphosphorylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-06-06 02:43:23ID requested
12015-05-12 02:12:10announced
Publication List
Henry C, Saadaoui B, Bouvier F, Cebo C, Phosphoproteomics of the goat milk fat globule membrane: New insights into lipid droplet secretion from the mammary epithelial cell. Proteomics, 15(13):2307-17(2015) [pubmed]
Keyword List
curator keyword: Biomedical, Biological
submitter keyword: milk, lipid droplet, phosphoproteome, secretion, mammary epithelial cell
Contact List
Christelle CEBO
contact affiliationINRA, UMR 1313 Unité Génétique Animale animale et Biologie Intégrative
contact emailchristelle.cebo@jouy.inra.fr
lab head
HENRY CELINE
contact affiliationINRA
contact emailceline.henry@jouy.inra.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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