PXD001033

PXD001033 is an original dataset announced via ProteomeXchange.

Title	in-depth phosphoproteomic profiling of mouse microglia BV-2 cell line
Description	We present a simple strategy (integrated Multistep enzyme digestion, Enrichment, Database search, “iMEED”) to improve coverage of the phosphoproteome from less starting material that is faster than other commonly used approaches. iMEED has generated the most comprehensive phosphoproteome of murine microglia to date, comprising 8807 unique phosphopeptides in 36 LC-MS/MS runs from 300 ug of starting material.
HostingRepository	PRIDE
AnnounceDate	2014-10-15
AnnouncementXML	Submission_2014-10-15_01:42:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dohyun Han
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-04 07:48:53	ID requested
⏵ 1	2014-10-15 01:42:10	announced

Han D, Jin J, Yu J, Kim K, Kim Y, Integrated approach using multistep enzyme digestion, TiO2 enrichment, and database search for in-depth phosphoproteomic profiling. Proteomics, 15(2-3):618-23(2015) [pubmed]

curator keyword: Biological

submitter keyword: Mouse, microglia, phosphoproteome, LC-MS/MS

Dohyun Han
contact affiliation	Colleage of Medicine, Seoul National University Seoul National University Hospital
contact email	hdh03@snu.ac.kr
lab head
Dohyun Han
contact affiliation	Colleage of medicine
contact email	hdh03@snu.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/10/PXD001033

PRIDE project URI

• PRIDE
  1. PXD001033
     1. Label: PRIDE project
     2. Name: in-depth phosphoproteomic profiling of mouse microglia BV-2 cell line