PXD001026

PXD001026 is an original dataset announced via ProteomeXchange.

Title	Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome
Description	In targeted proteomics it is critical that peptides are not only proteotypic, but also accurately represent the level of the protein (quantotypic). Numerous approaches are used to identify proteotypic peptides, but quantotypic properties are rarely assessed. Here, we show that measuring ratios of proteotypic peptides across biological samples can be used to empirically identify peptides with good quantotypic properties, and use this to identify quantotypic peptides for 21% of the human kinome.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:09:31.475.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonathan Worboys
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Velos; TSQ Vantage

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-02 03:28:42	ID requested
1	2014-08-08 08:33:03	announced
2	2014-09-25 03:38:25	announced	Updated publication reference for PubMed record(s): 25152083.
⏵ 3	2024-10-22 04:09:32	announced	2024-10-22: Updated project metadata.

10.1038/nmeth.3072;

Worboys JD, Sinclair J, Yuan Y, J, ø, rgensen C, Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome. Nat Methods, 11(10):1041-4(2014) [pubmed]

curator keyword: Biomedical

submitter keyword: Quantotypic, Bioinformatics,SRM

Claus Jørgensen
contact affiliation	CRUK Manchester Institute
contact email	Claus.Jorgensen@cruk.manchester.ac.uk
lab head
Jonathan Worboys
contact affiliation	CRUK MI, ICR
contact email	jonathan.worboys@cruk.manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/08/PXD001026

PRIDE project URI

• PRIDE
  1. PXD001026
     1. Label: PRIDE project
     2. Name: Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome