PXD001011

PXD001011 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteome profiling of CNS-infiltrating autoreactive CD4+ cells reveals selective changes during experimental autoimmune encephalomyelitis
Description	Experimental autoimmune encephalomyelitis (EAE) is a murine model of multiple sclerosis, a chronic neurodegenerative and inflammatory autoimmune condition of the central nervous system (CNS). Pathology is driven by the infiltration of autoreactive CD4+ lymphocytes into the CNS where they attack neuronal sheaths causing ascending paralysis. We used an isotope-coded protein labelling approach to investigate the proteome of CD4+ cells isolated from the spinal cord and brain of mice at various stages of EAE progression in two EAE disease models; PLP139-151-induced relapsing-remitting EAE and MOG35-55-induced chronic EAE, which emulate the two forms of human multiple sclerosis. A total of 1120 proteins were quantified across disease onset, peak-disease and remission phases of disease and of these, 13 up-regulated proteins of interest were identified with functions relating to the regulation of inflammation, leukocyte adhesion and migration, tissue repair and the regulation of transcription/translation. Proteins implicated in processes such as inflammation (S100A4 and S100A9) and tissue repair (Annexin A1), which represent key events during EAE progression were validated by quantitative PCR. This is the first targeted analysis of autoreactive cells purified from the CNS during EAE, highlighting fundamental CD4+ cell-driven processes that occur during the initiation of relapse and remission stages of disease.
HostingRepository	PRIDE
AnnounceDate	2016-07-08
AnnouncementXML	Submission_2016-07-08_10:22:05.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001011
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lyron Winderbaum
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-pyridylethyl-L-cysteine; monohydroxylated residue; iodoacetamide derivatized residue; Bruker Daltonics SERVA-ICPL(TM) quantification chemistry: medium form - site K; Bruker Daltonics SERVA-ICPL(TM) quantification chemistry: heavy form; O-(L-isoaspartyl)-L-threonine (active site intermediate); Bruker Daltonics SERVA-ICPL(TM) quantification chemistry: medium form - site N-term; Bruker Daltonics SERVA-ICPL(TM) quantification chemistry; Bruker Daltonics SERVA-ICPL(TM) quantification chemistry: heavy form - site K
Instrument	ultraflex III TOF/TOF; ultrafleXtreme

Revision	Datetime	Status	ChangeLog Entry
0	2014-05-28 06:04:00	ID requested
⏵ 1	2016-07-08 10:22:07	announced

Turvey ME, Koudelka T, Comerford I, Greer JM, Carroll W, Bernard CC, Hoffmann P, McColl SR, Quantitative proteome profiling of CNS-infiltrating autoreactive CD4+ cells reveals selective changes during experimental autoimmune encephalomyelitis. J Proteome Res, 13(8):3655-70(2014) [pubmed]

curator keyword: Biomedical

submitter keyword: Autoimmunity, Experimental Autoimmune Encephalomyelitis (EAE), CD4+ T lymphocyte, Isotope Coded Protein Labelling (ICPL), MALDI-TOF/TOF.

Peter Hoffmann
contact affiliation	Adelaide Proteomics Centre
contact email	peter.hoffmann@adelaide.edu.au
lab head
Lyron Winderbaum
contact affiliation	University of Adelaide
contact email	lyron.winderbaum@student.adelaide.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001011
     1. Label: PRIDE project
     2. Name: Quantitative proteome profiling of CNS-infiltrating autoreactive CD4+ cells reveals selective changes during experimental autoimmune encephalomyelitis