PXD001005

PXD001005 is an original dataset announced via ProteomeXchange.

Title	Comparative phosphoproteomic analysis of mammalian renal glomeruli
Description	Glomerular biology is dependent on tightly controlled signal transduction networks that control phosphorylation of signaling proteins such as cytoskeletal regulators or slit diaphragm proteins of kidney podocytes. Recent, in-depth analysis of the mouse glomerular phosphoproteome by tandem mass spectrometry confidently identified thousands of phosphorylation sites providing a unique opportunity to study signaling in vivo. However, the multitude of phosphorylated residues demands for additional systems biology approaches to separate physiologically important sites from non-functional phosphorylated residues. Cross-species comparison of phosphorylation events is a powerful mean to functionally prioritize and identify physiologically meaningful phosphorylation sites. Here, we present the result of additional phosphoproteomic analyses of the glomerular phosphoproteome in cow and rat to allow cross-species comparisons. We discovered several phosphorylation sites with potentially high biological relevance, e.g. tyrosine phosphorylation of the cytoskeletal regulator synaptopodin and the slit diaphragm protein neph-1 (Kirrel). Moreover, cross-species comparisons revealed conserved phosphorylation of the slit diaphragm protein nephrin on an acidic cluster at the intracellular terminus and conserved podocin phosphorylation on the very carboxyl terminus of the protein. Given the pivotal role of podocin for glomerular biology we studied a highly conserved podocin phosphorylation site in greater detail and show that phosphorylation regulates affinity of the interaction with nephrin and CD2AP. Taken together, these results suggest that species comparisons of phosphoproteomic data may reveal regulatory principles in glomerular biology.
HostingRepository	PRIDE
AnnounceDate	2015-01-16
AnnouncementXML	Submission_2015-01-16_01:38:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Rinschen
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-05-23 08:42:33	ID requested
⏵ 1	2015-01-16 01:38:43	announced

Rinschen MM, Pahmeyer C, Pisitkun T, Schnell N, Wu X, Maa, ß M, Bartram MP, Lamkemeyer T, Schermer B, Benzing T, Brinkkoetter PT, Comparative phosphoproteomic analysis of mammalian glomeruli reveals conserved podocin C-terminal phosphorylation as a determinant of slit diaphragm complex architecture. Proteomics, 15(7):1326-31(2015) [pubmed]

curator keyword: Biological

submitter keyword: Bovine, Rat, Kidney, Glomerulus, Phosphoproteome, Conservation

Markus Rinschen
contact affiliation	University of Cologne, CECAD
contact email	markus.rinschen@uk-koeln.de
lab head
Markus Rinschen
contact affiliation	Kidney research center cologne
contact email	markus.rinschen@uk-koeln.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001005
     1. Label: PRIDE project
     2. Name: Comparative phosphoproteomic analysis of mammalian renal glomeruli