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PXD001004

PXD001004 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of LMTK3 protein by LC/MS/MS Analysis of 1D SDS PAGE Gel Bands
DescriptionIdentification of LMTK3 by LC/MS/MS. A plasmid encoding for full-length LMTK3 was overexpressed in MCF-7 breast cancer cells. Following antibody detection by WB, two bands of interest were seen at around 200 kDa and 150 kDa on the blotting membrane. Colloidal Coomassie (CCBB) staining was performed on a separate gel, to which the Western blot was aligned with the gel markers and a region of gel corresponding to the bands of interest were excised, trypsin digested and analysed by LC/MS/MS.
HostingRepositoryPRIDE
AnnounceDate2022-02-22
AnnouncementXMLSubmission_2022-02-22_03:36:54.844.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD001004
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterGeorgios Giamas
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListPhospho; Oxidation; Carbamidomethyl
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-05-23 08:33:54ID requested
12022-02-22 03:36:55announced
Publication List
Xu Y, Zhang H, Lit LC, Grothey A, Athanasiadou M, Kiritsi M, Lombardo Y, Frampton AE, Green AR, Ellis IO, Ali S, Lenz HJ, Thanou M, Stebbing J, Giamas G, . Sci Signal, 7(330):ra58(2014) [pubmed]
Keyword List
submitter keyword: LMTK3, SILAC, GRB2, INTEGRIN B1, BREAST CANCER
Contact List
Georgios Giamas
contact affiliationDivision of Surgery and Cancer Imperial College, London
contact emailg.giamas@imperial.ac.uk
lab head
Georgios Giamas
contact affiliationImperial College
contact emailg.giamas@imperial.ac.uk
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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