PXD001004

PXD001004 is an original dataset announced via ProteomeXchange.

Title	Identification of LMTK3 protein by LC/MS/MS Analysis of 1D SDS PAGE Gel Bands
Description	Identification of LMTK3 by LC/MS/MS. A plasmid encoding for full-length LMTK3 was overexpressed in MCF-7 breast cancer cells. Following antibody detection by WB, two bands of interest were seen at around 200 kDa and 150 kDa on the blotting membrane. Colloidal Coomassie (CCBB) staining was performed on a separate gel, to which the Western blot was aligned with the gel markers and a region of gel corresponding to the bands of interest were excised, trypsin digested and analysed by LC/MS/MS.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_03:36:54.844.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001004
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Georgios Giamas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Phospho; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2014-05-23 08:33:54	ID requested
⏵ 1	2022-02-22 03:36:55	announced

Xu Y, Zhang H, Lit LC, Grothey A, Athanasiadou M, Kiritsi M, Lombardo Y, Frampton AE, Green AR, Ellis IO, Ali S, Lenz HJ, Thanou M, Stebbing J, Giamas G, . Sci Signal, 7(330):ra58(2014) [pubmed]

submitter keyword: LMTK3, SILAC, GRB2, INTEGRIN B1, BREAST CANCER

Georgios Giamas
contact affiliation	Division of Surgery and Cancer Imperial College, London
contact email	g.giamas@imperial.ac.uk
lab head
Georgios Giamas
contact affiliation	Imperial College
contact email	g.giamas@imperial.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD001004

PRIDE project URI

• PRIDE
  1. PXD001004
     1. Label: PRIDE project
     2. Name: Identification of LMTK3 protein by LC/MS/MS Analysis of 1D SDS PAGE Gel Bands