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PXD001002

PXD001002 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEgg yolk proteins G. fossarum
DescriptionWe aimed to specifically identify the major egg yolk proteins (i.e. vitellogenin) in the amphipod Gammarus fossarum, a sentinel species in freshwater risk assessment. Vitellogenin production in female is vital for the embryonic development and its production in male organism is commonly employed as a biomarker of exposition to xenoestrogens in fish. We performed a shotgun proteomics analysis on embryos and ovaries at different stages of their development. The proteome dynamics over the cycle was analyzed to correlate on the first hand proteins which are accumulating along vitellogenesis and on the other hand, proteins with decrease amounts long embryogenesis. This information led to the proposal of putative novel VTG candidates. Our data provides the first large scale molecular description of a crustacean reproductive cycle, while our temporal analysis evidenced seven candidates proteins as “true vitellogenin” in G. fossarum.
HostingRepositoryPRIDE
AnnounceDate2016-07-15
AnnouncementXMLSubmission_2016-07-15_08:51:40.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD001002
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJean ARMENGAUD
SpeciesList scientific name: Gammarus fossarum; NCBI TaxID: 52638;
ModificationListOxidation
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-05-23 03:35:29ID requested
12016-07-15 08:51:45announced
Publication List
Trapp J, Armengaud J, Gaillard JC, Pible O, Chaumot A, Geffard O, High-throughput proteome dynamics for discovery of key proteins in sentinel species: Unsuspected vitellogenins diversity in the crustacean Gammarus fossarum. J Proteomics, 146():207-14(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Amphipod, reproduction, vitellogenin, proteogenomics
Contact List
Jean Armengaud
contact affiliationCEA-Marcoule Institute of Environnemental Biology and Biotechnology Department of Biochemistry and Nuclear Toxicology Biochemistry of Perturbed Systems Laboratory France
contact emailjean.armengaud@cea.fr
lab head
Jean ARMENGAUD
contact affiliationSBTN
contact emailjean.armengaud@cea.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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