PXD000993

PXD000993 is an original dataset announced via ProteomeXchange.

Title	Virion proteome of Cafeteria roenbergensis virus strain BV-PW1
Description	This project describes the protein composition of the Cafeteria roenbergensis virus (CroV) particle, a giant marine DNA virus that infects the heterotrophic nanoflagellate microeukaryote C. roenbergensis. CroV is a member of the Nucleo-Cytoplasmic Large DNA Virus clade and related to Acanthamoeba polyphaga mimivirus. CroV possesses a DNA genome of ~730 kilobase pairs that encodes 544 predicted proteins. We analyzed the protein composition of purified CroV particles by liquid chromatography - tandem mass spectrometry (LC-MS/MS) and identified 141 virion-associated CroV proteins. Predicted functions could be assigned to 37% of these proteins, which include structural proteins as well as enzymes for transcription, DNA repair, redox reactions and protein modification. Homologs of 36 CroV virion proteins have previously been found in the virion of Acanthamoeba polyphaga mimivirus. This study shows that giant DNA virus particles contain more than one hundred viral proteins that include specific enzymatic functions.
HostingRepository	PRIDE
AnnounceDate	2014-05-22
AnnouncementXML	Submission_2014-05-22_02:38:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthias Fischer
SpeciesList	scientific name: Cafeteria roenbergensis; NCBI TaxID: 33653;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-05-21 02:50:00	ID requested
⏵ 1	2014-05-22 02:38:53	announced

Dataset with its publication pending

submitter keyword: LC-MSMS, virion proteome, CroV, capsid, giant virus, virophage

Leonard Foster
contact affiliation	Biochemistry & Molecular Biology, University of British Columbia, Vancouver, BC, Canada
contact email	foster@chibi.ubc.ca
lab head
Matthias Fischer
contact affiliation	Max Planck Institute for Medical Research, Heidelberg, Germany
contact email	mfischer@mpimf-heidelberg.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/05/PXD000993

PRIDE project URI

• PRIDE
  1. PXD000993
     1. Label: PRIDE project
     2. Name: Virion proteome of Cafeteria roenbergensis virus strain BV-PW1