PXD000990

PXD000990 is an original dataset announced via ProteomeXchange.

Title	Cocaine and Environmental Enrichment Proteomics Study of Rat Nucleus Accumbens
Description	Prior research demonstrated that environmental enrichment creates individual differences in behavior leading to a protective addiction phenotype in rats. Understanding the mechanisms underlying this phenotype will guide selection of targets for much-needed novel pharmacotherapeutics. The current study investigates basal differences in proteome expression in the nucleus accumbens of enriched and isolated rats and the proteomic response to cocaine self-administration using a liquid chromatography mass spectrometry (LCMS) technique to quantify 1917 proteins. Results of complementary Ingenuity Pathways Analyses (IPA) and gene set enrichment analyses (GSEA) demonstrate that cocaine increases vesicular transporters for dopamine and glutamate, as well as increasing proteins in the RhoA pathway. Further, cocaine regulates proteins related to ERK, CREB and AKT signaling. Environmental enrichment altered expression of a large number of proteins with diverse functions, including mood disorders, energy production, ubiquitination, splicing, transport, kinases and neurodegenerative diseases. Most of the biological functions and pathways listed above were also identified in the Cocaine X Enrichment interaction analysis, providing clear evidence that enriched and isolated rats respond quite differently to cocaine exposure. The overall impression of the current results is that enriched saline-administering rats have a unique proteomic complement compared to enriched cocaine-administering rats as well as saline and cocaine-taking isolated rats. These results identify possible mechanisms of the protective phenotype and provide fertile soil for developing novel pharmacotherapeutics.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_03:55:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cheryl Lichti
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-05-20 02:50:22	ID requested
1	2014-07-22 05:00:24	announced
2	2014-07-24 05:18:01	announced	Updated project metadata.
3	2014-08-08 05:58:38	announced	Updated project metadata.
⏵ 4	2014-10-23 03:55:31	announced	Updated project metadata.

Lichti CF, Fan X, English RD, Zhang Y, Li D, Kong F, Sinha M, Andersen CR, Spratt H, Luxon BA, Green TA, Environmental enrichment alters protein expression as well as the proteomic response to cocaine in rat nucleus accumbens. Front Behav Neurosci, 8():246(2014) [pubmed]

curator keyword: Biomedical

submitter keyword: Rat, Brain, Nucleus Accumbens, LC-MS/MS, Label-free Quantification

Thomas Green
contact affiliation	Center for Addiction Research Department of Pharmacology and Toxicology The University of Texas Medical Branch Galveston, TX 77555
contact email	thomas.green@utmb.edu
lab head
Cheryl Lichti
contact affiliation	UTMB-Galvestion
contact email	cflichti@utmb.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/07/PXD000990

PRIDE project URI

• PRIDE
  1. PXD000990
     1. Label: PRIDE project
     2. Name: Cocaine and Environmental Enrichment Proteomics Study of Rat Nucleus Accumbens