PXD000985

PXD000985 is an original dataset announced via ProteomeXchange.

Title	Quantitative phosphoproteomics analysis of olfactory receptor signaling in prostate cancer cells
Description	The olfactory receptor PSGR1 is not only expressed in olfactory tissue but shows expression in the prostate. In addition, the expression of this receptor is elevated in prostate cancer as well as other prostate diseases and can be used as diagnostic marker. The signalling cascade triggered by the activation of this receptor remains largely unknown until now. To investigate PSGR1 signalling, we stimulated the PSGR1 receptor with one of its known ligands, the odorant beta-ionone, and used a phosphoproteomics approach to identify mediators of PSGR1 signalling. We found the non-receptor tyrosine kinase Pyk2, the tumour suppressor NDRG1 as well as the sodium/hydrogen exchanger NHE1 to be downstream targets of activated PSGR1.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:20:53.482.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Friedel Drepper
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-05-19 03:31:13	ID requested
1	2016-07-08 11:27:48	announced
⏵ 2	2024-10-22 04:20:55	announced	2024-10-22: Updated project metadata.

Wiese H, Gelis L, Wiese S, Reichenbach C, Jovancevic N, Osterloh M, Meyer HE, Neuhaus EM, Hatt HH, Radziwill G, Warscheid B, Quantitative phosphoproteomics reveals the protein tyrosine kinase Pyk2 as a central effector of olfactory receptor signaling in prostate cancer cells. Biochim Biophys Acta, 1854(6):632-40(2015) [pubmed]

10.1016/j.bbapap.2014.09.002;

curator keyword: Biomedical

submitter keyword: NHE1,prostate cancer, Pyk2, PSGR1, ionone, phosphoproteomics

Bettina Warscheid
contact affiliation	Prof. Bettina Warscheid Funktionelle Proteomik Biologie II Albert-Ludwigs-Universität Freiburg Schänzlestr. 1 79104 Freiburg
contact email	bettina.warscheid@biologie.uni-freiburg.de
lab head
Friedel Drepper
contact affiliation	AG Warscheid Biologie II Albert-Ludwigs-Universität Freiburg Schänzlestr. 1 79104 Freiburg Germany
contact email	friedel.drepper@biologie.uni-freiburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD000985

PRIDE project URI

• PRIDE
  1. PXD000985
     1. Label: PRIDE project
     2. Name: Quantitative phosphoproteomics analysis of olfactory receptor signaling in prostate cancer cells