<<< Full experiment listing

PXD000975

PXD000975 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFunction of CK2-alpha in a minimal circadian system
DescriptionCasein Kinase 2 (CK2) is an evolutionarily conserved kinase protein that phosphorylates a plethora of cellular target protein involved in processes including DNA repair, cell cycle control, and circadian rhythms. CK2 is functionally conserved across all eukaryotes, although the substrate proteins identified in targeted experiments on a diverse range of complex tissues are often different. A growing interest exists to identify conserved, core signalling principles essential to eukaryotic life. To efficiently study generic roles of CK2 in the cellular circadian clock, we used a minimal eukaryotic model organism (Ostreococcus tauri). Overexpression of CK2 leads to a slow circadian rhythm, verifying functional conservation of CK2 in cellular timekeeping. CK2 activity contributes more strongly to the clock around dusk than around dawn, and analysis of the phospho-proteome at this time revealed a strong overrepresentation of potential CK2 sites. Large changes to the phospho-proteome are reported here upon overexpression of CK2, identifying a set of CK2-responsive phospho-sites. To identify more CK2-specific phosphorylation events, the phospho-proteome of cells treated with CK2 inhibitor were analysed and compared to the overexpression lines. We thus report a full inventory of the CK2-responsive phospho-proteome to inform studies into CK2 activity in the circadian clock of more complex tissues, as science moves to understand and modulate cellular circadian rhythms to help us negate the negative effects of shiftwork or cross-continental travel.
HostingRepositoryPRIDE
AnnounceDate2015-08-11
AnnouncementXMLSubmission_2015-08-11_02:24:03.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000975
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterThierry Le Bihan
SpeciesList scientific name: Ostreococcus tauri; NCBI TaxID: 70448;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; phosphorylated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-05-15 02:48:23ID requested
12015-08-11 02:24:04announced
Publication List
Le Bihan T, Hindle M, Martin SF, Barrios-Llerena ME, Krahmer J, Kis K, Millar AJ, van Ooijen G, Label-free quantitative analysis of the casein kinase 2-responsive phosphoproteome of the marine minimal model species Ostreococcus tauri. Proteomics, 15(23-24):4135-44(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Ostreococcus tauri, phosphopeptide quantitation, label-free analysis
Contact List
Thierry Le bihan
contact affiliationUniversity of Edinburgh
contact emailthierry.lebihan@ed.ac.uk
lab head
Thierry Le Bihan
contact affiliationCentre for Systems biology at edinburgh
contact emailthierry.lebihan@ed.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD000975
PRIDE project URI
Repository Record List
[ + ]