PXD000975 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Function of CK2-alpha in a minimal circadian system |
Description | Casein Kinase 2 (CK2) is an evolutionarily conserved kinase protein that phosphorylates a plethora of cellular target protein involved in processes including DNA repair, cell cycle control, and circadian rhythms. CK2 is functionally conserved across all eukaryotes, although the substrate proteins identified in targeted experiments on a diverse range of complex tissues are often different. A growing interest exists to identify conserved, core signalling principles essential to eukaryotic life. To efficiently study generic roles of CK2 in the cellular circadian clock, we used a minimal eukaryotic model organism (Ostreococcus tauri). Overexpression of CK2 leads to a slow circadian rhythm, verifying functional conservation of CK2 in cellular timekeeping. CK2 activity contributes more strongly to the clock around dusk than around dawn, and analysis of the phospho-proteome at this time revealed a strong overrepresentation of potential CK2 sites. Large changes to the phospho-proteome are reported here upon overexpression of CK2, identifying a set of CK2-responsive phospho-sites. To identify more CK2-specific phosphorylation events, the phospho-proteome of cells treated with CK2 inhibitor were analysed and compared to the overexpression lines. We thus report a full inventory of the CK2-responsive phospho-proteome to inform studies into CK2 activity in the circadian clock of more complex tissues, as science moves to understand and modulate cellular circadian rhythms to help us negate the negative effects of shiftwork or cross-continental travel. |
HostingRepository | PRIDE |
AnnounceDate | 2015-08-11 |
AnnouncementXML | Submission_2015-08-11_02:24:03.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD000975 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Thierry Le Bihan |
SpeciesList | scientific name: Ostreococcus tauri; NCBI TaxID: 70448; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; phosphorylated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-05-15 02:48:23 | ID requested | |
⏵ 1 | 2015-08-11 02:24:04 | announced | |
Publication List
Le Bihan T, Hindle M, Martin SF, Barrios-Llerena ME, Krahmer J, Kis K, Millar AJ, van Ooijen G, Label-free quantitative analysis of the casein kinase 2-responsive phosphoproteome of the marine minimal model species Ostreococcus tauri. Proteomics, 15(23-24):4135-44(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Ostreococcus tauri, phosphopeptide quantitation, label-free analysis |
Contact List
Thierry Le bihan |
contact affiliation | University of Edinburgh |
contact email | thierry.lebihan@ed.ac.uk |
lab head | |
Thierry Le Bihan |
contact affiliation | Centre for Systems biology at edinburgh |
contact email | thierry.lebihan@ed.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD000975 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000975
- Label: PRIDE project
- Name: Function of CK2-alpha in a minimal circadian system