PXD000973

PXD000973 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic analysis of calcium signaling
Description	Calcium signaling plays a pivotal role in t cell activation through activation of kinases and phosphatases, but comprehensive analysis to overview calcium-induced phosphorylation has never been performed. To investigate Ca2+-mediated phosphorylation globally, we next undertook a phosphoproteomic analysis of calcium signaling, especially focused on the Ca2+-dependent phosphatase, calcineurin. Normal mouse thymocytes were stimulated in 4 different conditions; 1). DMSO, 2). CsA, 3). IOM, or 4). CsA and IOM, and compared their phosphorylation status.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_03:45:43.996.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000973
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Atsushi Hatano
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Phospho; iTRAQ4plex; Glu->pyro-Glu; Oxidation; Carbamidomethyl; Gln->pyro-Glu
Instrument	QSTAR

Revision	Datetime	Status	ChangeLog Entry
0	2014-05-14 08:33:13	ID requested
⏵ 1	2022-02-22 03:45:51	announced

Hatano A, Matsumoto M, Nakayama KI, Phosphoproteomics analyses show subnetwork systems in T-cell receptor signaling. Genes Cells, 21(10):1095-1112(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Calcium signaling, Calcineurin, Mouse thymocytes, iTRAQ

Keiichi I. Nakayama
contact affiliation	Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University
contact email	nakayak1@bioreg.kyushu-u.ac.jp
lab head
Atsushi Hatano
contact affiliation	Medical Institute of Bioregulation, Kyushu University
contact email	atsushi_hatano@bioreg.kyushu-u.ac.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000973
     1. Label: PRIDE project
     2. Name: Phosphoproteomic analysis of calcium signaling