PXD000967

PXD000967 is an original dataset announced via ProteomeXchange.

Title	Triple Null, a soybean bred to lack major seed allergen P34 and bioactive lectin and kunitz trypsin inhibitor
Description	Seeds, including soybean (Glycine max), contain bioactive proteins with anti-nutritional and immunological properties that affect metabolism and assimilation of seed derived nutrients and as allergens that can induce escalating adverse immune responses. The presence of anti-nutritional proteins requires soybean protein to be processed by heat that results in energy costs and alteration of soybean protein’s physiochemical properties. Nulls for bioactive seed proteins have been isolated from the USDA soybean collection including Kunitz trypsin inhibitor (TI) and soybean agglutinin (LE) and immunodominant soybean allergen P34 proteins. Each of these nulls has the potential to partially address concerns of soybean feed/food consumption and stacked together these traits can form a basis for engineering improved soybean cultivars. A stack of cultivars of recessive nulls of TI, LE, and P34 was created in a cv Williams 82 background termed “Triple Null”. The reduction of these proteins slightly diminishes the overall protein content of the seed that was analyzed by 2D IEF/SDS gels and proteomics. P34 and Kunitz Trypsin Inhibitor nulls are frame shift mutants shown by mass spectroscopy to accumulate small amounts of authentic protein. Triple Null’s capacity for biolistic transformation was assessed showing that Triple Null can be transformed permitting stacking of transgene traits. Triple Null has possible application as a conventional feed/food source and for immunotherapy to mitigate soybean allergenic response and be leveraged as a transformation platform to stack other production or consumer traits.
HostingRepository	PRIDE
AnnounceDate	2022-02-23
AnnouncementXML	Submission_2022-02-23_08:54:42.339.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000967
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Eliot Herman
SpeciesList	scientific name: Glycine max (Soybean) (Glycine hispida); NCBI TaxID: 3847;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2014-05-12 03:58:36	ID requested
⏵ 1	2022-02-23 08:54:42	announced

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Allergen, null, seed, soybean

Eliot Herman
contact affiliation	School of Plant Sciences University of Arizona, USA
contact email	emherman@email.arizona.edu
lab head
Eliot Herman
contact affiliation	University of Arizona, Tucson
contact email	emherman@email.arizona.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000967
     1. Label: PRIDE project
     2. Name: Triple Null, a soybean bred to lack major seed allergen P34 and bioactive lectin and kunitz trypsin inhibitor