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PXD000960

PXD000960 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleK63 polyubiquitination is a new modulator of the oxidative stress response, shotgun data
DescriptionUbiquitination is a post-translational modification that signals multiple processes, including protein degradation, trafficking and DNA repair. Polyubiquitin accumulates globally during the oxidative stress response, and this has been mainly attributed to increased ubiquitin conjugation and perturbations in protein degradation. Here we show that the unconventional Lys 63 (K63)-linked polyubiquitin accumulates in the yeast Saccharomyces cerevisiae in a highly sensitive and regulated manner as a result of exposure to peroxides. We demonstrate that hydrogen peroxide inhibits the deubiquitinating enzyme Ubp2, leading to accumulation of K63 conjugates assembled by the Rad6 ubiquitin conjugase and the Bre1 ubiquitin ligase. Using linkage-specific isolation methods and stable isotope labeling by amino acids in cell culture (SILAC)–based quantitative proteomics, we identified >100 new K63-polyubiquitinated targets, which were substantially enriched in ribosomal proteins. Finally, we demonstrate that impairment of K63 ubiquitination during oxidative stress affects polysome stability and protein expression, rendering cells more sensitive to stress, and thereby reveal a new redox-regulatory role for this modification.
HostingRepositoryPRIDE
AnnounceDate2015-01-26
AnnouncementXMLSubmission_2015-01-27_02:35:09.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGustavo Silva
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-05-08 03:46:24ID requested
12015-01-26 02:13:05announced
22015-01-27 02:35:10announcedUpdated publication reference for PubMed record(s): 25622294.
Publication List
Silva GM, Finley D, Vogel C, K63 polyubiquitination is a new modulator of the oxidative stress response. Nat Struct Mol Biol, 22(2):116-23(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: K63 ubiquitination yeast oxidative stress
Contact List
Christine Vogel
contact affiliationCenter for Genomics and Systems Biology Department of Biology New York University, New York, NY, 10003, USA
contact emailcvogel@nyu.edu
lab head
Gustavo Silva
contact affiliationNew York University
contact emailgu_msilva@yahoo.com.br
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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