PXD000960 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | K63 polyubiquitination is a new modulator of the oxidative stress response, shotgun data |
Description | Ubiquitination is a post-translational modification that signals multiple processes, including protein degradation, trafficking and DNA repair. Polyubiquitin accumulates globally during the oxidative stress response, and this has been mainly attributed to increased ubiquitin conjugation and perturbations in protein degradation. Here we show that the unconventional Lys 63 (K63)-linked polyubiquitin accumulates in the yeast Saccharomyces cerevisiae in a highly sensitive and regulated manner as a result of exposure to peroxides. We demonstrate that hydrogen peroxide inhibits the deubiquitinating enzyme Ubp2, leading to accumulation of K63 conjugates assembled by the Rad6 ubiquitin conjugase and the Bre1 ubiquitin ligase. Using linkage-specific isolation methods and stable isotope labeling by amino acids in cell culture (SILAC)–based quantitative proteomics, we identified >100 new K63-polyubiquitinated targets, which were substantially enriched in ribosomal proteins. Finally, we demonstrate that impairment of K63 ubiquitination during oxidative stress affects polysome stability and protein expression, rendering cells more sensitive to stress, and thereby reveal a new redox-regulatory role for this modification. |
HostingRepository | PRIDE |
AnnounceDate | 2015-01-26 |
AnnouncementXML | Submission_2015-01-27_02:35:09.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gustavo Silva |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-05-08 03:46:24 | ID requested | |
1 | 2015-01-26 02:13:05 | announced | |
⏵ 2 | 2015-01-27 02:35:10 | announced | Updated publication reference for PubMed record(s): 25622294. |
Publication List
Silva GM, Finley D, Vogel C, K63 polyubiquitination is a new modulator of the oxidative stress response. Nat Struct Mol Biol, 22(2):116-23(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: K63 ubiquitination yeast oxidative stress |
Contact List
Christine Vogel |
contact affiliation | Center for Genomics and Systems Biology Department of Biology New York University, New York, NY, 10003, USA |
contact email | cvogel@nyu.edu |
lab head | |
Gustavo Silva |
contact affiliation | New York University |
contact email | gu_msilva@yahoo.com.br |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000960
- Label: PRIDE project
- Name: K63 polyubiquitination is a new modulator of the oxidative stress response, shotgun data