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PXD000959

PXD000959 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleN-linked glycoproteome of human seminal plasma
DescriptionN-linked glycosylation is an important type of post-translational modification. This modification plays important roles in many cellular functions including cell-cell and receptor-ligand interactions, immune response, apoptosis, and pathogenesis of many diseases. Seminal plasma is a mixture of secretions from several male accessory glands. Glycoproteins in seminal plasma can reflect the states of reproductive organs or diseases. Using isotope labeling and high-resolution mass spectrometry, we identified 720 N-glycosylated sites on 372 N-glycosylated proteins, corresponding to 365 genes. Analysis of variations among five individuals revealed similar compositions of N-glycosylated proteins in seminal plasma. Consistent composition of N-linked glycoproteins in human seminal plasma provides evidences that seminal plasma glycoproteins are of significant value to be candidates of biomarkers. Bioinformatics annotation of the identified seminal plasma glycoproteins showed that more than 43.0% of these glycoproteins are known secreted proteins according to UniProt. Such a high proportion of secreted glycoproteins in seminal plasma may play important roles in seminal plasma function such as making sure for sperm nutrition and function.
HostingRepositoryPRIDE
AnnounceDate2014-12-15
AnnouncementXMLSubmission_2014-12-15_08:22:12.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterzhou tao
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList(18)O labeled deglycosylated asparagine
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-05-08 03:37:20ID requested
12014-12-15 08:22:13announced
Publication List
Keyword List
curator keyword: Biomedical
submitter keyword: human,seminal plasma,glycoproteome,LC-MS/MS
Contact List
Jiahao Sha
contact affiliationState Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University
contact emailshajh@njmu.edu.cn
lab head
zhou tao
contact affiliationLife Sciences School
contact emailzhoutao@njmu.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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