PXD000951

PXD000951 is an original dataset announced via ProteomeXchange.

Title	29RNP kinase idetification
Description	The 29-kDa RNA protein (29RNP), which was originally identified in rice etioplasts, has increased phosphorylation levels during de-etiolation. Here, using heparin-sepharose enriched fractions, we identified a chloroplast kinase that phosphorylated 29RNP. The chloroplast kinase phosphorylated the 29RNP and exhibited CKII biochemical characteristics in a kinase reaction test. Proteomic analysis of the chloroplast heparin-sepharose enriched fractions revealed the presence of 70 proteins, including both abundant proteins, such as plastid encoded polymerase subunits, and low-abundance proteins, such as APO2 and DAG. An inclusion list method using Fourier transform ion cyclotron resonance mass spectrometer ( FT-ICR LTQ MS ) was subsequently used to analyze the chloroplast heparin-sepharose enriched fractions. The 29RNP kinase was positively identified as a CKII alpha family protein by the presence of two unique peptides.
HostingRepository	PRIDE
AnnounceDate	2015-04-27
AnnouncementXML	Submission_2015-04-27_03:43:28.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000951
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Qingtao Lu
SpeciesList	scientific name: Rice(O. sativa); NCBI TaxID: 4530;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2014-05-02 05:39:57	ID requested
⏵ 1	2015-04-27 03:43:29	announced

Lu Q, Ding S, Reiland S, R, ö, diger A, Roschitzki B, Xue P, Gruissem W, Lu C, Baginsky S, Identification and characterization of chloroplast casein kinase II from Oryza sativa (rice). J Exp Bot, 66(1):175-87(2015) [pubmed]

curator keyword: Biological

submitter keyword: 29RNP, kinase, m/z list inclusion method

Lu Qingtao
contact affiliation	Lu Qingtao, Ph.D. Photosynthesis Research Center, Room 230 Institute of Botany, CAS Haidian Xiangshan Nanxincun 20# Beijing. 100093 Phone, +86-10-62836254 Email, lu_qingtao@ibcas.ac.cn
contact email	lu_qingtao@ibcas.ac.cn
lab head
Qingtao Lu
contact affiliation	Ph.D
contact email	lu_qingtao@ibcas.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000951
     1. Label: PRIDE project
     2. Name: 29RNP kinase idetification