PXD009505

PXD009505 is an original dataset announced via ProteomeXchange.

Title	Use of lymph-circulating extracellular vesicles as surrogates of melanoma progression markers and BRAFV600E mutation.
Description	Liquid biopsies are becoming imperative on early patient diagnosis, prognosis and evaluation of residual disease. The use of circulating extracellular vesicles (EVs) as surrogate markers of tumor progression could be a powerful tool in the clinical setting. EVs in plasma have emerged as a non-invasive option to detect metastatic outcome, however sensitivity is low. Here we have characterized the lymph obtained after postoperative lymphadenectomy as a novel biological fluid enriched in EVs. Our proteomic profiling and BRAFV600E/K status determination demonstrate for the first time that EVs from the lymph of melanoma patients are enriched in melanoma-associated proteins and are useful for BRAF mutations detection. Melanoma oncogenic pathways, immunomodulation and platelet activating proteins are enriched in lymph-derived exosomes from patients with distal lymph node spread compared to local/early spreading. Furthermore, patients positive for BRAFV600E mutation on lymph-circulating vesicles had a shorter time of relapse. These data encourage the analysis of lymph-circulating EVs for detection of residual disease and recurrence.ADDENDUM: After the proper verification of the cell lines analysed in this dataset where it is written "SKMel103" or "SK103", it should be read as "SKMel147". This affects not only the raw files but also all the search results files. Sorry for the inconveniences.
HostingRepository	PRIDE
AnnounceDate	2021-02-26
AnnouncementXML	Submission_2021-02-26_06:55:55.117.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Javier Munoz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-16 01:37:52	ID requested
1	2019-03-13 12:19:04	announced
⏵ 2	2021-02-26 06:55:55	announced	2021-02-26: Updated publication reference for PubMed record(s): 30975894.

Garc, í, a-Silva S, Benito-Mart, í, n A, S, á, nchez-Redondo S, Hern, á, ndez-Barranco A, Xim, é, nez-Emb, ú, n P, Nogu, é, s L, Mazariegos MS, Brinkmann K, Amor L, ó, pez A, Meyer L, Rodr, í, guez C, Garc, í, a-Mart, í, n C, Boskovic J, Let, ó, n R, Montero C, Robledo M, Santambrogio L, Sue Brady M, Szumera-Cie, ć, kiewicz A, Kalinowska I, Skog J, Noerholm M, Mu, ñ, oz J, Ortiz-Romero PL, Ruano Y, Rodr, í, guez-Peralto JL, Rutkowski P, Peinado H, mutation. J Exp Med, 216(5):1061-1070(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Exosomes, Melanoma, Metastasis, Lymphatic fluid, BRAF

Javier Munoz
contact affiliation	CNIO
contact email	jmunozpe@cnio.es
lab head
Javier Munoz
contact affiliation	CNIO
contact email	jmunozpe@cnio.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD009505

PRIDE project URI

• PRIDE
  1. PXD009505
     1. Label: PRIDE project
     2. Name: Use of lymph-circulating extracellular vesicles as surrogates of melanoma progression markers and BRAFV600E mutation.