PXD000941 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Differences in proteasome targeting in Arabidopsis leaf mesophyll, epidermal and vascular cells |
Description | For the detailed and sensitive detection of the responses to a specific treatment it is important to perform tissue or cell type specific analyses, but this is not easily achievable for the different leaf tissues. Here we developed a method termed MeSelect to effectively separate leaf epidermis, vascular and mesophyll cells basically without contamination from other tissues. The high yield of the MeSelect method allowed for tissue specific high-throughput proteome analyses after inhibition of the proteasome with Syringolin A and the affinity enrichment of polyubiquitylated proteins in epidermis, mesophyll and vasculature. |
HostingRepository | PRIDE |
AnnounceDate | 2015-05-14 |
AnnouncementXML | Submission_2015-06-17_02:34:35.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD000941 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Katja Baerenfaller |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | iodoacetamide derivatized residue; oxidation |
Instrument | LTQ Orbitrap XL |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-04-29 10:29:22 | ID requested | |
1 | 2015-05-14 07:42:49 | announced | |
⏵ 2 | 2015-06-17 02:34:37 | announced | Updated publication reference for PubMed record(s): 26074939. |
Publication List
Svozil J, Gruissem W, Baerenfaller K, Proteasome targeting of proteins in Arabidopsis leaf mesophyll, epidermal and vascular tissues. Front Plant Sci, 6():376(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: ubiquitin proteasome system, Syringolin A, ubiquitylation, affinity enrichment, quantitative proteomics, Arabidopsis, leaf tissues, epidermis, vasculature, mesophyll |
Contact List
Katja Baerenfaller |
contact affiliation | ETH Zurich |
contact email | bkatja@ethz.ch |
lab head | |
Katja Baerenfaller |
contact affiliation | Institute of Plant Science |
contact email | bkatja@ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000941
- Label: PRIDE project
- Name: Differences in proteasome targeting in Arabidopsis leaf mesophyll, epidermal and vascular cells