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PXD000941

PXD000941 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDifferences in proteasome targeting in Arabidopsis leaf mesophyll, epidermal and vascular cells
DescriptionFor the detailed and sensitive detection of the responses to a specific treatment it is important to perform tissue or cell type specific analyses, but this is not easily achievable for the different leaf tissues. Here we developed a method termed MeSelect to effectively separate leaf epidermis, vascular and mesophyll cells basically without contamination from other tissues. The high yield of the MeSelect method allowed for tissue specific high-throughput proteome analyses after inhibition of the proteasome with Syringolin A and the affinity enrichment of polyubiquitylated proteins in epidermis, mesophyll and vasculature.
HostingRepositoryPRIDE
AnnounceDate2015-05-14
AnnouncementXMLSubmission_2015-06-17_02:34:35.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000941
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterKatja Baerenfaller
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListiodoacetamide derivatized residue; oxidation
InstrumentLTQ Orbitrap XL
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-04-29 10:29:22ID requested
12015-05-14 07:42:49announced
22015-06-17 02:34:37announcedUpdated publication reference for PubMed record(s): 26074939.
Publication List
Svozil J, Gruissem W, Baerenfaller K, Proteasome targeting of proteins in Arabidopsis leaf mesophyll, epidermal and vascular tissues. Front Plant Sci, 6():376(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: ubiquitin proteasome system, Syringolin A, ubiquitylation, affinity enrichment, quantitative proteomics, Arabidopsis, leaf tissues, epidermis, vasculature, mesophyll
Contact List
Katja Baerenfaller
contact affiliationETH Zurich
contact emailbkatja@ethz.ch
lab head
Katja Baerenfaller
contact affiliationInstitute of Plant Science
contact emailbkatja@ethz.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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