PXD000930

PXD000930 is an original dataset announced via ProteomeXchange.

Title	Naa30 is critically involved in maintaining mitochondrial and Golgi integrity
Description	N-terminal acetylation is one of the most common protein modifications in eukaryotes, with emerging roles in regulating protein quality and stoichiometry and targeting and complex formation. The NatC complex is one of the three major N-terminal acetyltransferases (NATs). Here, we partially defined the in vivo human NatC Nt-acetylome by combining siNAA30 mediated knockdown with positional proteomics. We identified 51 human NatC substrates and expanded our current knowledge on the substrate repertoire of NatC which now includes proteins harboring Met-Leu, Met-Ile, Met-Phe, Met-Trp, Met-Tyr, Met-Trp, Met-Met, Met-His and Met-Lys N-termini. Next to cytosolic proteins, several organellar proteins were identified as NatC substrates. Interestingly, upon Naa30 depletion, the levels of several organellar proteins were reduced, in particular those of mitochondrial proteins. Further, knockdown of Naa30 induced the loss of membrane potential, clearing and fragmentation of mitochondria. Naa30 depletion also led to disassembly of the Golgi apparatus. However, Naa30 depletion did not affect ER morphology, endosome or peroxisome distribution or microtubule and actin cytoskeletal architecture, suggesting that the observed mitochondrial fragmentation and clearance and Golgi scattering are not due to general disruption of organelles or microtubules. In conclusion, NatC Nt-acetylates a large variety of cytosolic and organellar proteins and is essential for cis-Golgi integrity and mitochondrial function.
HostingRepository	PRIDE
AnnounceDate	2016-10-05
AnnouncementXML	Submission_2016-10-05_05:20:43.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000930
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jonathan Vandenbussche
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C: 4x(15)N labeled L-arginine; S-carboxymethyl-L-cysteine: 58.005479; 2-pyrrolidone-5-carboxylic acid (Gln): -17.026549; N-acetylated residue: 42.010565; 3x(2)H labeled N6-acetyl-L-lysine: 45.029395; L-methionine (S)-sulfoxide: 15.994915
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-04-25 01:14:05	ID requested
⏵ 1	2016-10-05 05:20:44	announced

Van Damme P, Kalvik TV, Starheim KK, Jonckheere V, Myklebust LM, Menschaert G, Varhaug JE, Gevaert K, Arnesen T, A Role for Human N-alpha Acetyltransferase 30 (Naa30) in Maintaining Mitochondrial Integrity. Mol Cell Proteomics, 15(11):3361-3372(2016) [pubmed]

curator keyword: Biological

submitter keyword: human, Naa30, Golgi, Mitochondria, N-terminal COFRADIC

Kris Gevaert
contact affiliation	University Ghent, VIB
contact email	kris.gevaert@ugent.be
lab head
Jonathan Vandenbussche
contact affiliation	Biochemistry
contact email	jonathan.vandenbussche@vib-ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000930
     1. Label: PRIDE project
     2. Name: Naa30 is critically involved in maintaining mitochondrial and Golgi integrity