PXD000917

PXD000917 is an original dataset announced via ProteomeXchange.

Title	Proteomic identification of anti-tetanus toxoid antibodies from human serum (HD1)
Description	Proteomic identification and characterization of antibodies comprising the serological response to antigen can provide unique insight into the functional dynamics of adaptive immunity. We have developed a novel method to overcome the technical challenges which previously limited the direct analysis of immunoglobulin proteins in serum, as demonstrated by the identification of human anti-tetanus toxoid (TT) immunoglobulin G (IgG) proteins following booster vaccination. We analyzed the serum IgG repertoire across four time-points corresponding to pre-vaccination, 7 days, 3 months, and 9 months post vaccination. Antigen-specific antibodies were affinity purified against immobilized TT protein and sequenced by bottom-up nanoLC-MS/MS. Interpretation of mass spectra required a custom reference database of IgG heavy and light chain variable sequences determined by NextGen RNA sequencing of the donor's circulating plasmablasts and memory B cells following booster vaccination.
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-07-24_05:17:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Boutz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-04-22 02:28:37	ID requested
1	2014-04-24 03:08:13	announced
2	2014-04-24 12:26:09	announced	Updated publication reference for PubMed record(s): 24684310, 24469811.
⏵ 3	2014-07-24 05:17:18	announced	Updated project metadata.

Lavinder JJ, Wine Y, Giesecke C, Ippolito GC, Horton AP, Lungu OI, Hoi KH, DeKosky BJ, Murrin EM, Wirth MM, Ellington AD, D, ö, rner T, Marcotte EM, Boutz DR, Georgiou G, Identification and characterization of the constituent human serum antibodies elicited by vaccination. Proc Natl Acad Sci U S A, 111(6):2259-64(2014) [pubmed]

Boutz DR, Horton AP, Wine Y, Lavinder JJ, Georgiou G, Marcotte EM, Proteomic identification of monoclonal antibodies from serum. Anal Chem, 86(10):4758-66(2014) [pubmed]

curator keyword: Biomedical, Technical

submitter keyword: Antibody Proteomics, IgG, Human, LC-MSMS, Orbitrap Velos

Edward Marcotte
contact affiliation	Center for Systems and Synthetic Biology Institute for Cellular and Molecular Biology Department of Molecular Biosciences University of Texas at Austin
contact email	marcotte@icmb.utexas.edu
lab head
Daniel Boutz
contact affiliation	University of Texas at Austin
contact email	dboutz@austin.utexas.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/04/PXD000917

PRIDE project URI

• PRIDE
  1. PXD000917
     1. Label: PRIDE project
     2. Name: Proteomic identification of anti-tetanus toxoid antibodies from human serum (HD1)