PXD000916 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic identification of monoclonal antibodies from rabbit serum and analysis of FDR using differential cysteine labeling |
Description | Proteomic analysis of serum antibodies is challenging due to the non-germline origin of immunoglobulin (Ig) sequences and the complexity of Ig primary structure, with regions of hypervariable sequence interspersed within highly conserved framework sections resulting in an unusually high frequency of peptide-spectrum mis-identification using standard reference database search methods. Using a strategy of differential cysteine labeling with two alkylating reagents, we examined peptide-spectrum matches and decoy-based error modeling for the interpretation of antibody-derived mass spectral data. |
HostingRepository | PRIDE |
AnnounceDate | 2014-07-24 |
AnnouncementXML | Submission_2014-07-24_05:17:24.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Daniel Boutz |
SpeciesList | scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; S-(2-hydroxyethyl)cysteine |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-04-22 02:25:13 | ID requested | |
1 | 2014-04-24 02:44:32 | announced | |
⏵ 2 | 2014-07-24 05:17:25 | announced | Updated project metadata. |
Publication List
Keyword List
curator keyword: Biomedical, Technical |
submitter keyword: Antibody, IgG, Rabbit, LC-MSMS, Orbitrap Velos |
Contact List
Edward Marcotte |
contact affiliation | Center for Systems and Synthetic Biology Institute for Cellular and Molecular Biology Department of Molecular Biosciences University of Texas at Austin |
contact email | marcotte@icmb.utexas.edu |
lab head | |
Daniel Boutz |
contact affiliation | University of Texas at Austin |
contact email | dboutz@austin.utexas.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000916
- Label: PRIDE project
- Name: Proteomic identification of monoclonal antibodies from rabbit serum and analysis of FDR using differential cysteine labeling