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PXD000916

PXD000916 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic identification of monoclonal antibodies from rabbit serum and analysis of FDR using differential cysteine labeling
DescriptionProteomic analysis of serum antibodies is challenging due to the non-germline origin of immunoglobulin (Ig) sequences and the complexity of Ig primary structure, with regions of hypervariable sequence interspersed within highly conserved framework sections resulting in an unusually high frequency of peptide-spectrum mis-identification using standard reference database search methods. Using a strategy of differential cysteine labeling with two alkylating reagents, we examined peptide-spectrum matches and decoy-based error modeling for the interpretation of antibody-derived mass spectral data.
HostingRepositoryPRIDE
AnnounceDate2014-07-24
AnnouncementXMLSubmission_2014-07-24_05:17:24.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDaniel Boutz
SpeciesList scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; S-(2-hydroxyethyl)cysteine
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-04-22 02:25:13ID requested
12014-04-24 02:44:32announced
22014-07-24 05:17:25announcedUpdated project metadata.
Publication List
Keyword List
curator keyword: Biomedical, Technical
submitter keyword: Antibody, IgG, Rabbit, LC-MSMS, Orbitrap Velos
Contact List
Edward Marcotte
contact affiliationCenter for Systems and Synthetic Biology Institute for Cellular and Molecular Biology Department of Molecular Biosciences University of Texas at Austin
contact emailmarcotte@icmb.utexas.edu
lab head
Daniel Boutz
contact affiliationUniversity of Texas at Austin
contact emaildboutz@austin.utexas.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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