PXD000902

PXD000902 is an original dataset announced via ProteomeXchange.

Title	Global absolute quantification reveals tight regulation of protein expression in single Xenopus eggs
Description	While recent developments in genomic sequencing technology have enabled comprehensive transcriptome analyses of single cells, single cell proteomics has thus far been restricted to targeted studies. Here, we perform global absolute protein quantification of single Xenopus laevis eggs using mass spectrometry-based proteomics, quantifying over 5800 proteins in the largest single cell proteome characterized to date. Absolute protein amounts in single eggs are highly consistent, thus indicating a tight regulation of global protein abundance. In contrast, comparison between the single-cell proteome and transcriptome reveal poor expression correlation. Finally, we identify 439 proteins that significantly change in abundance during early embryogenesis. Many of these proteins do not show regulation at the transcript level. Altogether, our data reveal that the transcriptome is a poor indicator of the proteome and that protein levels are tightly controlled in Xenopus leavis eggs.
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-08-01_03:28:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	A.H. Smits
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-04-14 07:50:09	ID requested
1	2014-07-23 09:02:24	announced
2	2014-07-24 05:21:42	announced	Updated project metadata.
⏵ 3	2014-08-01 03:28:18	announced	Updated project metadata.

Smits AH, Lindeboom RG, Perino M, van Heeringen SJ, Veenstra GJ, Vermeulen M, Global absolute quantification reveals tight regulation of protein expression in single Xenopus eggs. Nucleic Acids Res, 42(15):9880-91(2014) [pubmed]

curator keyword: Biological

submitter keyword: Global absolute quantification reveals tight regulation of protein expression in single Xenopus eggs

Michiel Vermeulen
contact affiliation	Radboud University Nijmegen, Department of Molecular Biology, Cancer Genomics Netherlands, Faculty of Science, Radboud Institute for Molecular Life Sciences, Nijmegen 6500 HB, The Netherlands
contact email	m.vermeulen@ncmls.ru.nl
lab head
A.H. Smits
contact affiliation	University Medical Center Utrecht
contact email	a.smits-4@umcutrecht.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/07/PXD000902

PRIDE project URI

• PRIDE
  1. PXD000902
     1. Label: PRIDE project
     2. Name: Global absolute quantification reveals tight regulation of protein expression in single Xenopus eggs