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PXD000899

PXD000899 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCell cycle dependent phosphorylation of transmembrane proteins on the Theileria annulata schizont surface
DescriptionThe invasion of Theileria sporozoites into bovine leukocytes is rapidly followed by the liberation of the parasite from the parasitophorpous vacuole membrane, allowing the parasite to establish its niche within the host cell cytoplasm. Theileria infection induces host cell transformation, characterised by increased host cell proliferation and invasiveness, and the activation of anti-apoptotic genes. This process is strictly dependent on the presence of a viable parasite. Several host cell kinases, including PI3-K, PKA, CK2 and Src-family kinases, are known to be constitutively activated in Theileria infected cells and to contribute to the transformed phenotype. A number of host cell molecules, including the IB kinase and polo-like kinase 1 (Plk1), are recruited to the schizont surface, although very little is known about the schizont molecules involved. In this study we used immunofluorescence analysis to detect phosphorylated threonine (p-Thr and p-Thr-Pro) and serine (p-Ser) epitopes on the schizont during host cell cycle progression, revealing extensive schizont surface phosphorylation during host cell interphase. Further, we isolated schizonts from infected macrophages following synchronisation in S-phase or mitosis, and used mass spectrometry to detect phosphorylated schizont proteins. In total, 65 phosphorylated Theileria proteins were detected, 15 of which are potentially secreted or expressed on the surface of the schizont and thus mayight be targets forof host cell kinases. In particular, we describe the cell-cycle stage phosphorylation of two T. annulata surface expressed proteins, TaSP and p104. Phospho-peptides of both proteins were significantly more abundant in schizonts purified from S-phase cells.
HostingRepositoryPRIDE
AnnounceDate2014-12-12
AnnouncementXMLSubmission_2014-12-12_05:47:53.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000899
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDong Xia
SpeciesList scientific name: Theileria annulata; NCBI TaxID: 5874;
ModificationListdeamidated residue; monohydroxylated residue; iodoacetamide derivatized residue; phosphorylated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-04-14 01:08:10ID requested
12014-12-12 05:47:55announced
Publication List
Wiens O, Xia D, von Schubert C, Wastling JM, Dobbelaere DA, Heussler VT, Woods KL, Cell cycle-dependent phosphorylation of Theileria annulata schizont surface proteins. PLoS One, 9(7):e103821(2014) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Theileria annulata schizont, phosphorylation, Cell cycle dependent
Contact List
Jonathan Wastling
contact affiliationDepartment of Infection Biology Institute of Infection and Global Health University of Liverpool
contact emailwasj@liv.ac.uk
lab head
Dong Xia
contact affiliationInfection Biology
contact emaild.xia@liv.ac.uk
dataset submitter
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