PXD000899 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cell cycle dependent phosphorylation of transmembrane proteins on the Theileria annulata schizont surface |
Description | The invasion of Theileria sporozoites into bovine leukocytes is rapidly followed by the liberation of the parasite from the parasitophorpous vacuole membrane, allowing the parasite to establish its niche within the host cell cytoplasm. Theileria infection induces host cell transformation, characterised by increased host cell proliferation and invasiveness, and the activation of anti-apoptotic genes. This process is strictly dependent on the presence of a viable parasite. Several host cell kinases, including PI3-K, PKA, CK2 and Src-family kinases, are known to be constitutively activated in Theileria infected cells and to contribute to the transformed phenotype. A number of host cell molecules, including the IB kinase and polo-like kinase 1 (Plk1), are recruited to the schizont surface, although very little is known about the schizont molecules involved. In this study we used immunofluorescence analysis to detect phosphorylated threonine (p-Thr and p-Thr-Pro) and serine (p-Ser) epitopes on the schizont during host cell cycle progression, revealing extensive schizont surface phosphorylation during host cell interphase. Further, we isolated schizonts from infected macrophages following synchronisation in S-phase or mitosis, and used mass spectrometry to detect phosphorylated schizont proteins. In total, 65 phosphorylated Theileria proteins were detected, 15 of which are potentially secreted or expressed on the surface of the schizont and thus mayight be targets forof host cell kinases. In particular, we describe the cell-cycle stage phosphorylation of two T. annulata surface expressed proteins, TaSP and p104. Phospho-peptides of both proteins were significantly more abundant in schizonts purified from S-phase cells. |
HostingRepository | PRIDE |
AnnounceDate | 2014-12-12 |
AnnouncementXML | Submission_2014-12-12_05:47:53.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD000899 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Dong Xia |
SpeciesList | scientific name: Theileria annulata; NCBI TaxID: 5874; |
ModificationList | deamidated residue; monohydroxylated residue; iodoacetamide derivatized residue; phosphorylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-04-14 01:08:10 | ID requested | |
⏵ 1 | 2014-12-12 05:47:55 | announced | |
Publication List
Wiens O, Xia D, von Schubert C, Wastling JM, Dobbelaere DA, Heussler VT, Woods KL, Cell cycle-dependent phosphorylation of Theileria annulata schizont surface proteins. PLoS One, 9(7):e103821(2014) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Theileria annulata schizont, phosphorylation, Cell cycle dependent |
Contact List
Jonathan Wastling |
contact affiliation | Department of Infection Biology Institute of Infection and Global Health University of Liverpool |
contact email | wasj@liv.ac.uk |
lab head | |
Dong Xia |
contact affiliation | Infection Biology |
contact email | d.xia@liv.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000899
- Label: PRIDE project
- Name: Cell cycle dependent phosphorylation of transmembrane proteins on the Theileria annulata schizont surface