PXD000893

PXD000893 is an original dataset announced via ProteomeXchange.

Title	secretome study for human hepatic model during entamoeba invasion
Description	Investigations of human parasitic diseases depend on the availability of appropriate in vivo animal models and ex vivo experimental systems, and are particularly difficult for pathogens whose exclusive natural hosts are humans, as for Entamoeba histolytica, the protozoan parasite responsible for amoebiasis. We elaborated a human hepatic in vitro model consisting of cultured liver sinusoidal endothelial cells and hepatocytes in a 3D collagen-I environment. We have characterized the model's barrier function, its chemical and structural complexity and examined E. histolytica invasion. This study deals with secretome of hepatic model under various invasion conditions.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_03:12:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	gagan jhingan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Entamoeba histolytica HM-1:IMSS-A; NCBI TaxID: 885318; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-04-10 07:16:11	ID requested
1	2014-08-13 06:39:32	announced
⏵ 2	2014-10-23 03:12:16	announced	Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: hepatic cell, LSEC cell, entamoeba, Proteomics, Secretome

Nancy Guillen
contact affiliation	Cell Biology of Parasitism Unit Insitut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, FRANCE
contact email	nguillen@pasteur.fr
lab head
gagan jhingan
contact affiliation	NII
contact email	gaganjhingan@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/08/PXD000893

PRIDE project URI

• PRIDE
  1. PXD000893
     1. Label: PRIDE project
     2. Name: secretome study for human hepatic model during entamoeba invasion