PXD000891

PXD000891 is an original dataset announced via ProteomeXchange.

Title	Neisseria meningitides small RNA's regulate Citric acid cycle activity under Feast and Famine conditions
Description	We identified twin small non-coding RNAs regulating tricarboxylic acid (TCA) cycle activity in Neisseria meningitidis. Expression of TCA cycle enzymes was elevated in sRNA deletion mutants. Direct interaction between sRNAs and the ribosomal entry sites on target mRNAs was demonstrated. Expression of the sRNAs was down-regulated in cells grown in poor medium with glucose as the sole carbon source but not without lrp, indicating that sRNA expression is controlled by the stringent response. N. meningitidis, over-expressing the sRNAs replicated in blood, but not in human cerebrospinal fluid. In addition, Lrp synthesis was inhibited by direct interaction between the sRNA and the 5’ UTR of lrp. sRNAs control adaptation of N. meningitidis to variation in nutrient supply in different niches of its host.
HostingRepository	PRIDE
AnnounceDate	2017-03-22
AnnouncementXML	Submission_2017-03-27_01:31:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gertjan Kramer
SpeciesList	scientific name: Neisseria meningitidis serogroup B; NCBI TaxID: 491;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2014-04-10 05:23:01	ID requested
1	2017-03-22 05:07:05	announced
⏵ 2	2017-03-27 01:31:28	announced	Updated publication reference for PubMed record(s): 28325760.

Pannekoek Y, Huis In 't Veld RA, Schipper K, Bovenkerk S, Kramer G, Brouwer MC, van de Beek D, Speijer D, van der Ende A, Uses Sibling Small Regulatory RNAs To Switch from Cataplerotic to Anaplerotic Metabolism. mBio, 8(2):(2017) [pubmed]

curator keyword: Biological

submitter keyword: Neisseria meningitides, small RNA's, Feast and Famine, Data Independant Mass Spectrometry, Pathogens

Dave Speijer
contact affiliation	Clinical Proteomics Facility Medical Biochemistry Academic Medical Center, University of Amsterdam
contact email	d.speijer@amc.uva.nl
lab head
Gertjan Kramer
contact affiliation	Proteomics Unit
contact email	gertjan.kramer@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD000891

PRIDE project URI

• PRIDE
  1. PXD000891
     1. Label: PRIDE project
     2. Name: Neisseria meningitides small RNA's regulate Citric acid cycle activity under Feast and Famine conditions