PXD000887

PXD000887 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of the effect of Slf1 deletion on yeast response to oxidative stress via LC-MSMS
Description	The mechanisms by which RNA-binding proteins control the translation of subsets of mRNAs are not yet clear. Slf1p is an atypical La motif containing protein (LARP). LARPs are members of a superfamily of RNA-binding proteins conserved across eukaryotes. Transcriptomics and RIP-Seq analysis suggested an effect of Slf1 on the amount of mRNA of genes involved in the response to oxidative stress. To quantify these effects at the protein level, we used label-free mass spectrometry to compare the proteomes of wild-type and slf1Δ mutant strains following oxidative stress conditions. This analysis identified several proteins which are normally induced in response to hydrogen peroxide, but where this increase is attenuated in the slf1Δ mutant.
HostingRepository	PRIDE
AnnounceDate	2015-03-12
AnnouncementXML	Submission_2015-03-12_06:22:15.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000887
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Talavera
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	Oxidation; Oxidation; Carbamidomethyl
Instrument	Dionex instrument model; Mascot; Progenesis LC-MS; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2014-04-09 04:07:00	ID requested
⏵ 1	2015-03-12 06:22:16	announced

Kershaw CJ, Costello JL, Castelli LM, Talavera D, Rowe W, Sims PF, Ashe MP, Hubbard SJ, Pavitt GD, Grant CM, The yeast La related protein Slf1p is a key activator of translation during the oxidative stress response. PLoS Genet, 11(1):e1004903(2015) [pubmed]

submitter keyword: Yeast, Oxidative stress, Label-free quantification, LC-MSMS

Christopher Grant
contact affiliation	Faculty of Life Science, University of Manchester, United Kingdom
contact email	chris.grant@manchester.ac.uk
lab head
David Talavera
contact affiliation	University of Manchester
contact email	David.Talavera@manchester.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000887
     1. Label: PRIDE project
     2. Name: Proteomics analysis of the effect of Slf1 deletion on yeast response to oxidative stress via LC-MSMS