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PXD000883

PXD000883 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRBDmap
DescriptionThe RNA interactomes of HeLa and HEK293 cells jointly comprise 1106 RNA-binding proteins (RBPs), with almost half of these lacking well-defined RNA-binding domains (RBDs), suggesting the existence of numerous unknown RNA-binding architectures. Here, we report on “RBDmap”, a new method built on interactome capture, to comprehensively identify the RBDs of native RBPs in HeLa cells. Making use of in vivo UV-crosslinking of RBPs to polyadenylated RNAs, capture on oligo(dT) magnetic beads, sequential proteolytic digestion and LC-MS/MS combined with a sophisticated scoring algorithm, RBDmap “re-discoveres” many known RNA-binding sites (e.g. RRM, KH) of numerous well characterized RBPs and suggests novel RBDs.
HostingRepositoryPRIDE
AnnounceDate2016-08-11
AnnouncementXMLSubmission_2016-08-11_00:32:04.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristian Frese
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-04-09 00:47:40ID requested
12016-08-09 07:12:11announced
22016-08-11 00:32:07announcedUpdated project metadata.
Publication List
Castello A, Fischer B, Frese CK, Horos R, Alleaume AM, Foehr S, Curk T, Krijgsveld J, Hentze MW, Comprehensive Identification of RNA-Binding Domains in Human Cells. Mol Cell, 63(4):696-710(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: RNA-binding proteins, RNA binding domain
Contact List
Jeroen Krijgsveld
contact affiliationEuropean Molecular Biology Laboratory, Genome Biology Unit Meyerhofstr. 1 69117 Heidelberg Germany
contact emailkrijgsveld@embl.de
lab head
Christian Frese
contact affiliationDKFZ German Cancer Research Center
contact emailfrese@embl.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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