PXD000880

PXD000880 is an original dataset announced via ProteomeXchange.

Title	Biochemical profile of the A.thaliana hypusination pathway
Description	The eukaryotic translation elongation factor eIF5A is the only protein known to contain the unusual amino acid hypusine which is essential for its biological activity. This posttranslational modification is achieved by the sequential action of the enzymes deoxyhypusine synthase and deoxyhypusine hydroxylase. The crucial molecular function of eIF5A during translation has been recently elucidated in yeast and it is expected to be fully conserved in every eukaryotic cell, however the functional description of this pathway in plants is still scarce. The genetic approaches with transgenic plants for either eIF5A overexpression or antisense have revealed some activities related to the control of cell death processes but the molecular details remain to be characterized. One important aspect to fully understand this pathway is the biochemical description of the hypusine modification system. Here we have used recombinant eIF5A proteins either modified by hypusination or non-modified to establish a bi-dimensional electrophoresis (2D-E) profile for the three eIF5A protein isoforms present in Arabidopsis thaliana. The combined use of the recombinant 2D-E profile together with 2D-E/western blot analysis from whole plant extracts has provided a quantitative approach to measure the hypusination status of eIF5A. We have used this information to demonstrate that treatment with the hormone abscisic acid produces an alteration of the hypusine modification system in Arabidopsis thaliana. Overall this study presents the first biochemical description of the posttranslational modification of eIF5A by hypusination which will be functionally relevant for future studies related to the characterization of this pathway in Arabidopsis thaliana.
HostingRepository	PRIDE
AnnounceDate	2016-07-09
AnnouncementXML	Submission_2016-07-08_16:48:12.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000880
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sergio Ciordia
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2014-04-07 08:06:09	ID requested
⏵ 1	2016-07-08 16:48:14	announced

Belda-Palaz, ó, n B, Nohales MA, Rambla JL, Ace, ñ, a JL, Delgado O, Fustero S, Mart, í, nez MC, Granell A, Carbonell J, Ferrando A, Biochemical quantitation of the eIF5A hypusination in Arabidopsis thaliana uncovers ABA-dependent regulation. Front Plant Sci, 5():202(2014) [pubmed]

submitter keyword: spermidine, eIF5A, hypusine, abscisic acid, 2D-electrophoresis

Dr. Alejandro Ferrando
contact affiliation	Instituto de Biología Molecular y Celular de Plantas, CSIC-Universidad Politécnica de Valencia, Valencia, Spain. Tel: +34-963879931
contact email	aferrando@ibmcp.upv.es
lab head
Sergio Ciordia
contact affiliation	National Center for Biotechnology
contact email	sciordia@cnb.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000880
     1. Label: PRIDE project
     2. Name: Biochemical profile of the A.thaliana hypusination pathway