PXD000879

PXD000879 is an original dataset announced via ProteomeXchange.

Title	Discovery proteomics on cutaneous biopsies
Description	Lyme disease is the most important vector-borne disease in the Northern hemisphere and represents a major public health challenge. The disease diagnosis relies mainly on clinical practice that can be coupled to serological or molecular diagnostics. However the relatively low sensitivity and specificity of the available Lyme diagnostics indicate the urgent need to identify additional highly antigenic borrelial proteins or to develop new strategies of diagnostic. As the skin constitutes a key interface where the pathogens can persist and multiply, we investigated proteomics on skin samples to detect Borrelia proteins directly in cutaneous biopsies in a robust and specific way. We first set up a discovery Ge-LC-MS/MS approach on a murine model infected by B. burgdorferi sensu stricto. This dataset contains all the results concerning this approach.
HostingRepository	PRIDE
AnnounceDate	2016-07-09
AnnouncementXML	Submission_2016-07-08_17:14:00.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000879
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Schnell Gilles
SpeciesList	scientific name: Borrelia burgdorferi 297; NCBI TaxID: 521009;
ModificationList	iodoacetamide -site C: 57.021464; acetylated residue: 42.010565; monohydroxylated residue: 15.994915; N-acetylated residue: 42.010565
Instrument	ion trap

Revision	Datetime	Status	ChangeLog Entry
0	2014-04-04 04:59:42	ID requested
⏵ 1	2016-07-08 17:14:01	announced

Schnell G, Boeuf A, Westermann B, Jaulhac B, Lipsker D, Carapito C, Boulanger N, Ehret-Sabatier L, Discovery and targeted proteomics on cutaneous biopsies infected by borrelia to investigate lyme disease. Mol Cell Proteomics, 14(5):1254-64(2015) [pubmed]

submitter keyword: Lyme borreliosis, skin interface, LC-MS/MS

Laurence Sabatier
contact affiliation	IPHC-DSA-LSMBO-UMR 7178
contact email	laurence.sabatier@unistra.fr
lab head
Schnell Gilles
contact affiliation	IPHC
contact email	gilles.schnell@etu.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD000879

PRIDE project URI

• PRIDE
  1. PXD000879
     1. Label: PRIDE project
     2. Name: Discovery proteomics on cutaneous biopsies