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PXD000878

PXD000878 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphoproteomics of MuSK Signaling
DescriptionThe development of the neuromuscular synapse depends on signaling processes which involve protein phosphorylation as a crucial regulatory event. The receptor tyrosine kinase MuSK is the key signaling molecule at the neuromuscular synapse whose activity is required for the formation of a mature and functional neuromuscular synapse. However, the signaling cascade downstream of MuSK and the regulation of the different components is still poorly understood. Here we present data from a quantitative phosphoproteomics approach to study MuSK-dependent processes. Muscle cells were stimulated with the heparansulfate proteoglycan agrin, which activates MuSK and downstream signaling events. To get an insight into the signaling dynamics we used three different time points (unstimulated, 15 min, 60 min and 4 hrs).
HostingRepositoryPRIDE
AnnounceDate2014-07-24
AnnouncementXMLSubmission_2014-07-24_05:16:11.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000878
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterGerhard Duernberger
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; phosphorylated residue; iTRAQ4plex-116 reporter+balance reagent acylated residue; methylthiolated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-04-04 03:36:07ID requested
12014-06-05 09:33:03announced
22014-06-06 02:02:15announcedUpdated publication reference for PubMed record(s): 24899341.
32014-07-24 05:16:12announcedUpdated project metadata.
Publication List
D, ΓΌ, rnberger G, Camurdanoglu BZ, Tomschik M, Schutzbier M, Roitinger E, Hudecz O, Mechtler K, Herbst R, Global analysis of muscle-specific kinase signaling by quantitative phosphoproteomics. Mol Cell Proteomics, 13(8):1993-2003(2014) [pubmed]
Keyword List
curator keyword: Biomedical, Biological
submitter keyword: MuSK, muscle, phosphoproteomics, time course, iTRAQ
Contact List
Karl Mechtler
contact affiliationIMP - Research Institute of Molecular Pathology, IMBA - Institute of Molecular Biotechnology
contact emailkarl.mechtler@imp.ac.at
lab head
Gerhard Duernberger
contact affiliationGMI/IMP/IMBA
contact emailgerhard.duernberger@imp.ac.at
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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