PXD000878 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteomics of MuSK Signaling |
Description | The development of the neuromuscular synapse depends on signaling processes which involve protein phosphorylation as a crucial regulatory event. The receptor tyrosine kinase MuSK is the key signaling molecule at the neuromuscular synapse whose activity is required for the formation of a mature and functional neuromuscular synapse. However, the signaling cascade downstream of MuSK and the regulation of the different components is still poorly understood. Here we present data from a quantitative phosphoproteomics approach to study MuSK-dependent processes. Muscle cells were stimulated with the heparansulfate proteoglycan agrin, which activates MuSK and downstream signaling events. To get an insight into the signaling dynamics we used three different time points (unstimulated, 15 min, 60 min and 4 hrs). |
HostingRepository | PRIDE |
AnnounceDate | 2014-07-24 |
AnnouncementXML | Submission_2014-07-24_05:16:11.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD000878 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Gerhard Duernberger |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; phosphorylated residue; iTRAQ4plex-116 reporter+balance reagent acylated residue; methylthiolated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-04-04 03:36:07 | ID requested | |
1 | 2014-06-05 09:33:03 | announced | |
2 | 2014-06-06 02:02:15 | announced | Updated publication reference for PubMed record(s): 24899341. |
⏵ 3 | 2014-07-24 05:16:12 | announced | Updated project metadata. |
Publication List
D, ΓΌ, rnberger G, Camurdanoglu BZ, Tomschik M, Schutzbier M, Roitinger E, Hudecz O, Mechtler K, Herbst R, Global analysis of muscle-specific kinase signaling by quantitative phosphoproteomics. Mol Cell Proteomics, 13(8):1993-2003(2014) [pubmed] |
Keyword List
curator keyword: Biomedical, Biological |
submitter keyword: MuSK, muscle, phosphoproteomics, time course, iTRAQ |
Contact List
Karl Mechtler |
contact affiliation | IMP - Research Institute of Molecular Pathology, IMBA - Institute of Molecular Biotechnology |
contact email | karl.mechtler@imp.ac.at |
lab head | |
Gerhard Duernberger |
contact affiliation | GMI/IMP/IMBA |
contact email | gerhard.duernberger@imp.ac.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000878
- Label: PRIDE project
- Name: Phosphoproteomics of MuSK Signaling