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PXD000877

PXD000877 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIn-depth proteomic analysis of carp (Cyprinus carpio L) spermatozoa
DescriptionUsing a combination of protein fractionation by one-dimensional gel electrophoresis and high performance liquid chromatography - electrospray ionization tandem mass spectrometry, we identified 348 proteins in carp spermatozoa, most of which were for the first time identified in fish. Dynein, tubulin, HSP90, HSP70, HSP60, adenosylhomocysteinase, NKEF-B, brain type creatine kinase, mitochondrial ATP synthase, and valosin containing enzyme represent high abundance proteins in carp spermatozoa. These proteins are functionally related to sperm motility and energy production as well as the protection of sperm against oxidative injury and stress. Moreover, carp sperm is equipped with functionally diverse proteins involved in signal transduction, transcription, translation, protein turnover and transport. About 15% of carp sperm proteins identified here were also detected in seminal plasma which may be a result of leakage from spermatozoa into seminal plasma, adsorption of seminal plasma proteins on spermatozoa surface, and expression in both spermatozoa and cells secreting seminal plasma proteins. The availability of a catalogue of carp sperm proteins provides substantial advances for an understanding of sperm function and for future development of molecular diagnostic tests of carp sperm quality, the evaluation of which is currently limited to certain parameters such as sperm count, morphology and motility or viability.
HostingRepositoryPRIDE
AnnounceDate2014-10-13
AnnouncementXMLSubmission_2014-10-13_02:19:08.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000877
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMariola Dietrich
SpeciesList scientific name: Cyprinus carpio (Common carp); NCBI TaxID: 7962;
ModificationListmonohydroxylated residue
InstrumentLTQ
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-04-04 01:12:01ID requested
12014-10-13 02:19:09announced
Publication List
Dietrich MA, Arnold GJ, Fr, รถ, hlich T, Ciereszko A, In-depth proteomic analysis of carp (Cyprinus carpio L) spermatozoa. Comp Biochem Physiol Part D Genomics Proteomics, 12():10-5(2014) [pubmed]
Keyword List
submitter keyword: carp, sperm, proteome, LC-MS/MS, fish
Contact List
Mariola A. Dietrich
contact affiliationInstitute of Animal Reproduction and Food Research, Polish Academy of Sciences, Department of Gamete and Embryo Biology, Olsztyn, Poland
contact emailm.dietrich@pan.olsztyn.pl
lab head
Mariola Dietrich
contact affiliationPolish Academy of Sciences
contact emailm.dietrich@pan.olsztyn.pl
dataset submitter
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Dataset FTP location
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