PXD000877 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | In-depth proteomic analysis of carp (Cyprinus carpio L) spermatozoa |
Description | Using a combination of protein fractionation by one-dimensional gel electrophoresis and high performance liquid chromatography - electrospray ionization tandem mass spectrometry, we identified 348 proteins in carp spermatozoa, most of which were for the first time identified in fish. Dynein, tubulin, HSP90, HSP70, HSP60, adenosylhomocysteinase, NKEF-B, brain type creatine kinase, mitochondrial ATP synthase, and valosin containing enzyme represent high abundance proteins in carp spermatozoa. These proteins are functionally related to sperm motility and energy production as well as the protection of sperm against oxidative injury and stress. Moreover, carp sperm is equipped with functionally diverse proteins involved in signal transduction, transcription, translation, protein turnover and transport. About 15% of carp sperm proteins identified here were also detected in seminal plasma which may be a result of leakage from spermatozoa into seminal plasma, adsorption of seminal plasma proteins on spermatozoa surface, and expression in both spermatozoa and cells secreting seminal plasma proteins. The availability of a catalogue of carp sperm proteins provides substantial advances for an understanding of sperm function and for future development of molecular diagnostic tests of carp sperm quality, the evaluation of which is currently limited to certain parameters such as sperm count, morphology and motility or viability. |
HostingRepository | PRIDE |
AnnounceDate | 2014-10-13 |
AnnouncementXML | Submission_2014-10-13_02:19:08.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD000877 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Mariola Dietrich |
SpeciesList | scientific name: Cyprinus carpio (Common carp); NCBI TaxID: 7962; |
ModificationList | monohydroxylated residue |
Instrument | LTQ |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-04-04 01:12:01 | ID requested | |
⏵ 1 | 2014-10-13 02:19:09 | announced | |
Publication List
Dietrich MA, Arnold GJ, Fr, รถ, hlich T, Ciereszko A, In-depth proteomic analysis of carp (Cyprinus carpio L) spermatozoa. Comp Biochem Physiol Part D Genomics Proteomics, 12():10-5(2014) [pubmed] |
Keyword List
submitter keyword: carp, sperm, proteome, LC-MS/MS, fish |
Contact List
Mariola A. Dietrich |
contact affiliation | Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Department of Gamete and Embryo Biology, Olsztyn, Poland |
contact email | m.dietrich@pan.olsztyn.pl |
lab head | |
Mariola Dietrich |
contact affiliation | Polish Academy of Sciences |
contact email | m.dietrich@pan.olsztyn.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000877
- Label: PRIDE project
- Name: In-depth proteomic analysis of carp (Cyprinus carpio L) spermatozoa