PXD000876

PXD000876 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of three Borrelia burgdorferi sensu lato species and disseminating clones: relevance for Lyme vaccine design.
Description	Lyme borreliosis is first characterized by a cutaneous inflammation, the erythema migrans, and if dissemination occurs after an infected tick bite cutaneous, nervous and articular manifestations appear. Although antibiotic treatments are efficient in the early stage of the infection, a significant number of patients develop disseminated manifestations due to unnoticed or absence of erythema migrans, or to inappropriate treatment. Vaccine could be an efficient approach to decrease Lyme disease incidence. We have developed a proteomic approach based on a Ge-LC-MS/MS strategy to identify new vaccine candidates. We analyzed a disseminating clone and the associated wild type strain for each major pathogenic Borrelia species: B. burgdorferi sensu stricto, B. garinii and B. afzelii. We managed to identify proteins specific and common to the disseminating clones of the 3 main species. In parallel, we used a spectral counting strategy in order to identify up-regulated proteins common to the clones. Thus, we identified 40 proteins that are potentially involved in bacterial virulence and could be of interest in the development of a new vaccine.
HostingRepository	PRIDE
AnnounceDate	2015-03-20
AnnouncementXML	Submission_2015-03-20_01:31:23.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000876
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Schnell Gilles
SpeciesList	scientific name: Borrelia burgdorferi 297; NCBI TaxID: 521009;
ModificationList	iodoacetamide - site C: 57.021464; acetylated residue: 42.010565; monohydroxylated residue: 15.994915; N-acetylated residue: 42.010565
Instrument	ion trap

Revision	Datetime	Status	ChangeLog Entry
0	2014-04-04 01:00:10	ID requested
1	2015-02-16 01:40:26	announced
⏵ 2	2015-03-20 01:31:24	announced	Updated project metadata.

Schnell G, Boeuf A, Jaulhac B, Boulanger N, Collin E, Barthel C, De Martino S, Ehret-Sabatier L, Proteomic analysis of three Borrelia burgdorferi sensu lato native species and disseminating clones: relevance for Lyme vaccine design. Proteomics, 15(7):1280-90(2015) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Lyme borreliosis / Virulence / Comparative proteomics / Spectral counting

Laurence Sabatier
contact affiliation	IPHC-DSA-LSMBO-UMR7178 Strasbourg
contact email	laurence.sabatier@unistra.fr
lab head
Schnell Gilles
contact affiliation	IPHC
contact email	gilles.schnell@etu.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/02/PXD000876

PRIDE project URI

• PRIDE
  1. PXD000876
     1. Label: PRIDE project
     2. Name: Proteomic analysis of three Borrelia burgdorferi sensu lato species and disseminating clones: relevance for Lyme vaccine design.