PXD000863

PXD000863 is an original dataset announced via ProteomeXchange.

Title	Ion mobility tandem mass spectrometry enhances performance of bottom-up proteomics
Description	We describe a novel method utilizing ion mobility-based concentration of peptide fragment ions on a qTOF mass spectrometer improving the sensitivity of bottom-up proteomics by up to 10-fold. This enabled the identification of 7,500 human proteins within one day and 8,600 phosphorylation sites within 5h of LC-MS/MS time. The method also proved powerful for multiplexed quantification experiments exemplified by the chemoproteomic interaction analysis of HDACs with Trichostatin A.
HostingRepository	PRIDE
AnnounceDate	2015-08-18
AnnouncementXML	Submission_2015-08-18_08:45:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hannes Hahne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue; phosphorylated residue; TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2014-03-28 02:29:40	ID requested
⏵ 1	2015-08-18 08:45:44	announced

Helm D, Vissers JP, Hughes CJ, Hahne H, Ruprecht B, Pachl F, Grzyb A, Richardson K, Wildgoose J, Maier SK, Marx H, Wilhelm M, Becher I, Lemeer S, Bantscheff M, Langridge JI, Kuster B, Ion mobility tandem mass spectrometry enhances performance of bottom-up proteomics. Mol Cell Proteomics, 13(12):3709-15(2014) [pubmed]

curator keyword: Technical

submitter keyword: Ion mobility mass spectrometry, quadrupole time of flight mass spectrometer, proteomics, phosphoproteomics, chemical proteomics

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics Technische Universitaet Muenchen
contact email	kuster@tum.de
lab head
Hannes Hahne
contact affiliation	Chair of Proteomics and Bioanalytics
contact email	hannes.hahne@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD000863

PRIDE project URI

• PRIDE
  1. PXD000863
     1. Label: PRIDE project
     2. Name: Ion mobility tandem mass spectrometry enhances performance of bottom-up proteomics