PXD000852

PXD000852 is an original dataset announced via ProteomeXchange.

Title	Native protein mapping and visualization of protein interactions in human plasma high-density lipoprotein by 2-DE and LC-MS/MS
Description	A human plasma sample was subjected to non-denaturing micro 2-DE and a gel area (5 mm X 18 mm) which includes high-density lipoprotein (HDL) was cut into 1 mm X 1 mm squares, then the proteins in the 90 gel pieces were analyzed by in-gel trypsin digestion and nano-LC-MS/MS. Grid-cutting of the gel was employed to; 1) ensure the total analysis of the proteins in the area, 2) standardize the conditions of analysis by LC-MS/MS, 3) reconstruct the protein distribution patterns from the label-free quantification data. Totally 154 proteins (excluding keratins) were assigned in the 90 gel pieces and the quantity distribution of each was reconstructed as a color density pattern (a native protein map). The map of apolipoprotein (Apo) A-I showed a wide apparent mass distribution characteristic to HDL and was compared with the maps of the other 153 proteins. Eleven proteins showed maps of wide distribution that overlapped with the map of Apo A-I, and all have been reported to be the components of HDL. Further, seven minor proteins associated with HDL were detected at the gel positions of high Apo A-I quantity. These results for the first time visualized the localization of HDL apolipoproteins on a non-denaturing 2-DE gel and strongly suggested their interactions.
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-07-24_03:56:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ya Jin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2014-03-19 05:03:22	ID requested
1	2014-04-07 06:43:53	announced
⏵ 2	2014-07-24 03:56:07	announced	Updated project metadata.

Jin Y, Bu S, Zhang J, Yuan Q, Manabe T, Tan W, Native protein mapping and visualization of protein interactions in the area of human plasma high-density lipoprotein by combining nondenaturing micro 2DE and quantitative LC-MS/MS. Electrophoresis, 35(14):2055-64(2014) [pubmed]

curator keyword: Biological, Technical

submitter keyword: human, plasma, high-density lipoproteins, HDL, non-denaturing 2-DE, nano-LC-MS/MS, LC-MSE

Ya Jin
contact affiliation	School of Bioscience & Bioengineering, South China University of Technology
contact email	ya.jin@ymail.com
lab head
Ya Jin
contact affiliation	School of Bioscience and Biotechnology, South China University of Technology
contact email	ya.jin@ymail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/04/PXD000852

PRIDE project URI

• PRIDE
  1. PXD000852
     1. Label: PRIDE project
     2. Name: Native protein mapping and visualization of protein interactions in human plasma high-density lipoprotein by 2-DE and LC-MS/MS