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PXD000849

PXD000849 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCombined Antibody/Lectin-Enrichment Identifies Extensive Changes in the O-GlcNAc Subproteome Upon Oxidative Stress
DescriptionO-GlcNAc is thought to regulate proteins in a mannaer analogous to other PTMs, modulating cellular functions including the cellular stress response. The aim of this study was to identify specific cellular networks and protein complexes that are differentially O-GlcNAcylated and/or expressed upon acute oxidative stress (H2O2 for 1 and 2 h). We achieved this by employing SILAC and a novel anti-O-GlcNAc G5 lectibody IP strategy that combind O-GlcNAc specific antibodies and the lectin WGA. We identified 990 proteins among all three treatments including 202 non-redundant O-GlcNAc modified peptides. Differentially expressed proteins clustered into canonical 14-3-3/PI3K signaling pathways including the 14-3-3 complex, chaperonins, RNA Pol II Mediator and nuclear pore complexes.
HostingRepositoryPRIDE
AnnounceDate2016-09-29
AnnouncementXMLSubmission_2016-09-29_07:30:59.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlbert Lee
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListO-(N-acetylamino)hexosyl-L-serine; O-(N-acetylamino)hexosyl-L-threonine; 6x(13)C labeled residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-03-18 03:23:48ID requested
12016-09-29 07:31:00announced
Publication List
Lee A, Miller D, Henry R, Paruchuri VD, O'Meally RN, Boronina T, Cole RN, Zachara NE, Combined Antibody/Lectin Enrichment Identifies Extensive Changes in the O-GlcNAc Sub-proteome upon Oxidative Stress. J Proteome Res, 15(12):4318-4336(2016) [pubmed]
Keyword List
submitter keyword: Oxidative stress, O-GlcNAc, affinity chromatography, SILAC
Contact List
Natasha E Zachara
contact affiliationThe Johns Hopkins University, School of Medicine. The Department of Biological Chemistry.
contact emailnzachara@jhmi.edu
lab head
Albert Lee
contact affiliationMacquarie University
contact emailalbert.lee@mq.edu.au
dataset submitter
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Dataset FTP location
PRIDE project URI
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