PXD000848

PXD000848 is an original dataset announced via ProteomeXchange.

Title	iTRAQ-based quantitative proteomic analysis of membrane and nuclear fractions of glioma (WHO grade II, III and IV) tissues to map on Chromosome 12 and identification of mass spectrometry evidence for 'missing proteins'
Description	Glioma tumors arise from normal glial cells (astrocytes). Gliomas are morphologically and clinically classified into four malignancy grades as Grade I, II, III and IV. Grade I and II comprise low grade gliomas that grow slowly, are well differentiated and have a better prognosis and survival compared to grades III and IV. In contrast, grade III-IV lack endothelial proliferation and are highly vascular with a tendency to infiltrate and have areas of extensive necrosis and hypoxia. In the present study, we used iTRAQ (isobaric tags for relative and absolute quantitation) - based quantitative proteomic approach followed by liquid chromatography and high resolution tandem mass spectrometry (LC-MS/MS) to identify differential membrane and nuclear proteins mapping to Chromosome 12 from three different grades of astrocytoma tumors (WHO Grade II, III and IV) compared to control.
HostingRepository	PRIDE
AnnounceDate	2019-06-04
AnnouncementXML	Submission_2019-06-04_04:19:23.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ravi Sirdeshmukh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; methylthiolated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-03-18 02:08:05	ID requested
⏵ 1	2019-06-04 04:19:24	announced

Gupta MK, Jayaram S, Madugundu AK, Chavan S, Advani J, Pandey A, Thongboonkerd V, Sirdeshmukh R, Chromosome-centric human proteome project: deciphering proteins associated with glioma and neurodegenerative disorders on chromosome 12. J Proteome Res, 13(7):3178-90(2014) [pubmed]

submitter keyword: Glioma, diffused astrocytoma, anaplastic astrocytoma, Glioblastoma, sub cellular fractionation, membrane proteins, nuclear proteins, iTRAQ, Chromosome 12, synaptic vesicle 2-related protein, IQ motif containing D

Dr. Ravi Sirdeshmukh
contact affiliation	Institute of Bioinformatics, Bangalore - 560066, India
contact email	ravisirdeshmukh@gmail.com
lab head
Ravi Sirdeshmukh
contact affiliation	Institute of Bioinformatics
contact email	ravisirdeshmukh@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000848
     1. Label: PRIDE project
     2. Name: iTRAQ-based quantitative proteomic analysis of membrane and nuclear fractions of glioma (WHO grade II, III and IV) tissues to map on Chromosome 12 and identification of mass spectrometry evidence for 'missing proteins'