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PXD000840

PXD000840 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMRM qualifcation of secreted proteins differentially expressed in Brucella-infected macrophage cells vs non-infected macrophage cells
DescriptionProtein biomarkers that are secreted into the blood from infected host cells following infection may become the basis of early and specific disease diagnostics. To identify such mechanistic biomarkers, the human macrophage cell line THP-1 was infected with Brucella abortus 2308 or 544 for 20 hours. Light membrane vesicles were prepared from infected and control cultures and the secretory protein-enriched soluble content recovered for proteomics analysis. Statistical analysis identified 184 significantly differentially expressed proteins from the infected macrophages. Upregulated proteins were used for MRM qualication. One hundred forty four (144) peptides corresponding to 65 proteins were monitored.
HostingRepositoryPeptideAtlas
AnnounceDate2014-03-18
AnnouncementXMLSubmission_2014-03-18_09:55:52.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterEustache Paramithiotis
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
Instrument4000 Q-Trap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-03-17 09:35:03ID requested
12014-03-18 09:55:52announced
Publication List
no publication
Keyword List
curator keyword: selected reaction monitoring, SRM, targeted
Contact List
Eustache Paramithiotis
contact affiliation
contact emaileparamithiotis@caprion.com
dataset submitter
Eustache Paramithiotis
contact affiliationCaprion.com
contact emaileparamithiotis@caprion.com
lab head
Full Dataset Link List
PeptideAtlas dataset URI
PASSEL experiment URI
PASSEL transition group browser URI