PXD000840
PXD000840 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | MRM qualifcation of secreted proteins differentially expressed in Brucella-infected macrophage cells vs non-infected macrophage cells |
Description | Protein biomarkers that are secreted into the blood from infected host cells following infection may become the basis of early and specific disease diagnostics. To identify such mechanistic biomarkers, the human macrophage cell line THP-1 was infected with Brucella abortus 2308 or 544 for 20 hours. Light membrane vesicles were prepared from infected and control cultures and the secretory protein-enriched soluble content recovered for proteomics analysis. Statistical analysis identified 184 significantly differentially expressed proteins from the infected macrophages. Upregulated proteins were used for MRM qualication. One hundred forty four (144) peptides corresponding to 65 proteins were monitored. |
HostingRepository | PeptideAtlas |
AnnounceDate | 2014-03-18 |
AnnouncementXML | Submission_2014-03-18_09:55:52.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Eustache Paramithiotis |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | 4000 Q-Trap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2014-03-17 09:35:03 | ID requested | |
⏵ 1 | 2014-03-18 09:55:52 | announced |
Publication List
no publication |
Keyword List
curator keyword: selected reaction monitoring, SRM, targeted |
Contact List
Eustache Paramithiotis | |
---|---|
contact affiliation | |
contact email | eparamithiotis@caprion.com |
dataset submitter | |
Eustache Paramithiotis | |
contact affiliation | Caprion.com |
contact email | eparamithiotis@caprion.com |
lab head |
Full Dataset Link List
PeptideAtlas dataset URI |
PASSEL experiment URI |
PASSEL transition group browser URI |