PXD000837

PXD000837 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of plasma membrane and tonoplast from the leaves of a mangrove plant Avicennia officinalis
Description	We used gel electrophoresis coupled with LC-MS based proteomics to identify key transport proteins in the plasma membrane (PM) and tonoplast fractions of Avicennia officinalis leaves. This was part of our attempts to understand salt tolerance and secretion in mangrove plant species. PM and tonoplast proteins were purified using two-aqueous phase partitioning and density gradient centrifugation, respectively. Thirty of the 154 PM proteins and 31 of the 118 tonoplast proteins identified were predicted to have transmembrane domains. About 90% of the identified proteins could be classified based on their functions. The major classes of proteins were predicted to be involved in transport, metabolic processes, signal transduction and defense /stress response, while a few of the proteins were predicted to be involved in membrane trafficking. The main classes of transporter proteins identified included H+-ATPases, ATP-binding cassette transporters (ABC) and aquaporins, all of which could play a role in salt secretion. These data will serve as the baseline membrane proteomic dataset for Avicennia species. Further, this information can contribute to future studies on understanding the mechanism of salt tolerance in halophytes in addition to salt secretion in mangroves.
HostingRepository	PRIDE
AnnounceDate	2014-11-19
AnnouncementXML	Submission_2014-11-19_00:30:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qingsong Lin
SpeciesList	scientific name: Avicennia; NCBI TaxID: 41377;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2014-03-17 04:51:35	ID requested
1	2014-11-18 01:27:34	announced
2	2014-11-18 08:29:59	announced	Updated publication details.
⏵ 3	2014-11-19 00:30:44	announced	Updated project metadata.

Krishnamurthy P, Tan XF, Lim TK, Lim TM, Kumar PP, Loh CS, Lin Q, Proteomic analysis of plasma membrane and tonoplast from the leaves of mangrove plant Avicennia officinalis. Proteomics, 14(21-22):2545-57(2014) [pubmed]

curator keyword: Biological

submitter keyword: Plasma membrane, Tonoplast, Proteomics, Avicennia officinalis, salt secretion

Assistant Professor Lin Qingsong
contact affiliation	National University of Singapore, Department of Biological Science, Singapore
contact email	dbslinqs@nus.edu.sg
lab head
Qingsong Lin
contact affiliation	Biological Sciences
contact email	dbslinqs@nus.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/11/PXD000837

PRIDE project URI

• PRIDE
  1. PXD000837
     1. Label: PRIDE project
     2. Name: Proteomic analysis of plasma membrane and tonoplast from the leaves of a mangrove plant Avicennia officinalis