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PXD000834

PXD000834 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePositional proteomics identification of the natural substrates of cytosolic carboxypeptidases
DescriptionCytosolic carboxypeptidases (CCPs) constitute a new subfamily of M14 metallocarboxypeptidases associated to axonal regeneration and neuronal degeneration, amongst others. CCPs are deglutamylating enzymes, able to catalyze the shortening of polyglutamate side-chains and the gene-encoded C-termini of tubulin, telokin and myosin light chain kinase. The functions of these enzymes are not entirely understood, in part due to lack of information about C-terminal protein processing in the cell and its functional implications. By means of C-terminal COFRADIC, a positional proteomics approach, we searched for cellular substrates targets of CCP1, the most relevant member of this family. We here identified 7 new putative CCP1 protein substrates, including ribosomal proteins, translation factors and high mobility group proteins. 
HostingRepositoryPRIDE
AnnounceDate2014-12-02
AnnouncementXMLSubmission_2014-12-02_04:56:56.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000834
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSebastian Tanco
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList4x(2)H labeled alpha-dimethylamino N-terminal residue: 32.056407; 2-pyrrolidone-5-carboxylic acid (Gln): -17.026549; 3x(2)H labeled N6-acetyl-L-lysine: 45.029395; N-acetylated residue: 42.010565; L-methionine (S)-sulfoxide: 15.994915; acetate labeling reagent (N-term) (heavy form: +3amu)
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-03-17 03:28:15ID requested
12014-12-02 04:56:57announced
Publication List
Tanco S, Tort O, Demol H, Aviles FX, Gevaert K, Van Damme P, Lorenzo J, C-terminomics screen for natural substrates of cytosolic carboxypeptidase 1 reveals processing of acidic protein C termini. Mol Cell Proteomics, 14(1):177-90(2015) [pubmed]
Keyword List
ProteomeXchange project tag: PRIME-XS Project
curator keyword: Biological
submitter keyword: degradomics C-terminomics carboxypeptidase
Contact List
Kris Gevaert
contact affiliationVIB Department of Medical Protein Research, UGent Proteome Analysis and Bioinformatics Unit B9000 Gent, Belgium
contact emailKris.Gevaert@vib-ugent.be
lab head
Sebastian Tanco
contact affiliationDepartment of Medical Protein Research, VIB-Ghent University
contact emailsebastian.tanco@vib-ugent.be
dataset submitter
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Dataset FTP location
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