PXD000833

PXD000833 is an original dataset announced via ProteomeXchange.

Title	MRM qualifcation of secreted proteins in Brucella-infected/non-infected trophoblast cell lines
Description	Protein biomarkers that are secreted into the blood from infected host cells following infection may become the basis of early and specific disease diagnostics. To identify such mechanistic biomarkers, two human trophoblast cell lines (JAr and AC-1M88) were infected with Brucella abortus 2308 for 20 hours. Light membrane vesicles were prepared from infected and control cultures and the secretory protein-enriched soluble content recovered for proteomics analysis. Statistical analysis identified 287 and 143 significantly differentially expressed proteins from each of the infected JAr and AC-1M88 trophoblast preparations respetively. Proteins selected from each cell line were used for MRM qualication. Two hundred on (201) peptides corresponding to 113 proteins were monitored. Seventy four (74) proteins were qualified as candidate biomarkers of Brucella diagnosis
HostingRepository	PeptideAtlas
AnnounceDate	2014-03-18
AnnouncementXML	Submission_2014-03-18_09:55:51.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Eustache Paramithiotis
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	4000 Q-Trap

Revision	Datetime	Status	ChangeLog Entry
0	2014-03-14 12:27:32	ID requested
⏵ 1	2014-03-18 09:55:52	announced

no publication

curator keyword: selected reaction monitoring, SRM, targeted

Eustache Paramithiotis
contact affiliation
contact email	eparamithiotis@caprion.com
dataset submitter
Eustache Paramithiotis
contact affiliation	Caprion.com
contact email	eparamithiotis@caprion.com
lab head

PeptideAtlas dataset URI

PASSEL experiment URI

PASSEL transition group browser URI