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PXD000821

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2014-07-24
  • AnnouncementXML: Submission_2014-07-24_05:16:02.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Sara Zanivan
  • Title: In vivo SILAC-based proteomics reveals phosphoproteome changes during mouse skin carcinogenesis
  • Description: Cancer progresses through distinct stages and mouse models recapitulating traits of this progression are frequently used to explore genetic, morphological and pharmacological aspects of tumor development. To complement genomic investigations of this process, here we quantify phosphoproteomic changes in skin cancer development using the SILAC mouse technology coupled to high resolution mass spectrometry. We distill protein expression signatures from our data that distinguish between skin cancer stages. A distinct phosphoproteome of the two stages of cancer progression is identified which correlates with perturbed cell growth and implicates cell adhesion as a major driver of malignancy. Importantly, integrated analysis of phosphoproteomic data and prediction of kinase activity revealed PAK4-PKC/SRC network to be highly deregulated in SCC but not in papilloma. This detailed molecular picture, both at the proteome and phosphoproteome level, will prove useful for the study of mechanisms of tumor progression.
  • SpeciesList: scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
  • ModificationList: phosphorylated residue; 6x(13)C labeled residue
  • Instrument: LTQ Orbitrap Velos; LTQ Orbitrap Elite

Dataset History

VersionDatetimeStatusChangeLog Entry
02014-03-13 03:25:25ID requested
12014-03-13 07:11:04announced
22014-07-24 05:16:03announcedUpdated project metadata.

Publication List

  1. Zanivan S, Meves A, Behrendt K, Schoof EM, Neilson LJ, Cox J, Tang HR, Kalna G, van Ree JH, van Deursen JM, Trempus CS, Machesky LM, Linding R, Wickström SA, Fässler R, Mann M, In vivo SILAC-based proteomics reveals phosphoproteome changes during mouse skin carcinogenesis. Cell Rep, 3(2):552-66(2013) [pubmed]

Keyword List

  1. curator keyword: Biomedical
  2. submitter keyword: SILAC, skin carcinogenesis, phosphoproteomics, mouse

Contact List

    Sara Zanivan
    • contact affiliation: Cancer Research UK Beatson Institute
    • contact email: s.zanivan@beatson.gla.ac.uk
    • lab head:
    Sara Zanivan
    • contact affiliation: Vascular Proteomics
    • contact email: s.zanivan@beatson.gla.ac.uk
    • dataset submitter:

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