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PXD000806

PXD000806 is an original dataset announced via ProteomeXchange.

Dataset Summary
Titlewheat seed phosphoproteome under water deficit
DescriptionDrought is one of the most serious abiotic stresses, under which the crop yield is significantly reduced. Elite winter wheat cultivar Henong 341 (Triticum aestivum L.) was grown in experimental fields of China Hebei province (116°37′23″E, 37°41′02″N) Two different treatment conditions were designed: rain-fed (no water irrigation) and well-watered (water irrigation) conditions at jointing and flowering stages. Each treatment included three biological replicates and each plot was 25m2. In this study, a comprehensive phosphoproteomic analysis of developing grains of 26th day after flowering (DAF) in Chinese bread wheat cultivar Henong 341 under well-watered and water-deficit conditions was performed by means of TiO2 enrichment, LC/MS/MS analysis, and Label-free peptide-intensity quantification. Under well-watered conditions, a total of 590 unique phosphopeptides corresponding to 603 nonredundant phosphorylation sites, representing 471 phosphoproteins, were identified. While under water deficit, 63 unique phosphopeptides, corresponding to 61 phosphoproteins, were found to be differently regulated in phosphorylation status compared to well-watered conditions (≥2 fold intensities).
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:19:46.914.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterming zhang
SpeciesList scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationListphosphorylated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-03-10 02:59:07ID requested
12016-07-08 18:55:06announced
22024-10-22 04:19:47announced2024-10-22: Updated project metadata.
Publication List
10.1021/pr500400t;
Zhang M, Ma CY, Lv DW, Zhen SM, Li XH, Yan YM, Comparative phosphoproteome analysis of the developing grains in bread wheat (Triticum aestivum L.) under well-watered and water-deficit conditions. J Proteome Res, 13(10):4281-97(2014) [pubmed]
Keyword List
submitter keyword: drought response and defense, phosphoproteome,bread wheat, water stress, seed development
Contact List
Ming Zhang
contact affiliationCollege of Life Sciences, Capital Normal University No. 105 Xisanhuan North Road, Beijing 100048 P. R. China
contact emailzhangming5152007@163.com
lab head
ming zhang
contact affiliationschool of life sciences
contact emailzhangming5152007@163.com
dataset submitter
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Dataset FTP location
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