PXD000806 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | wheat seed phosphoproteome under water deficit |
Description | Drought is one of the most serious abiotic stresses, under which the crop yield is significantly reduced. Elite winter wheat cultivar Henong 341 (Triticum aestivum L.) was grown in experimental fields of China Hebei province (116°37′23″E, 37°41′02″N) Two different treatment conditions were designed: rain-fed (no water irrigation) and well-watered (water irrigation) conditions at jointing and flowering stages. Each treatment included three biological replicates and each plot was 25m2. In this study, a comprehensive phosphoproteomic analysis of developing grains of 26th day after flowering (DAF) in Chinese bread wheat cultivar Henong 341 under well-watered and water-deficit conditions was performed by means of TiO2 enrichment, LC/MS/MS analysis, and Label-free peptide-intensity quantification. Under well-watered conditions, a total of 590 unique phosphopeptides corresponding to 603 nonredundant phosphorylation sites, representing 471 phosphoproteins, were identified. While under water deficit, 63 unique phosphopeptides, corresponding to 61 phosphoproteins, were found to be differently regulated in phosphorylation status compared to well-watered conditions (≥2 fold intensities). |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:19:46.914.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | ming zhang |
SpeciesList | scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-03-10 02:59:07 | ID requested | |
1 | 2016-07-08 18:55:06 | announced | |
⏵ 2 | 2024-10-22 04:19:47 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1021/pr500400t; |
Zhang M, Ma CY, Lv DW, Zhen SM, Li XH, Yan YM, Comparative phosphoproteome analysis of the developing grains in bread wheat (Triticum aestivum L.) under well-watered and water-deficit conditions. J Proteome Res, 13(10):4281-97(2014) [pubmed] |
Keyword List
submitter keyword: drought response and defense, phosphoproteome,bread wheat, water stress, seed development |
Contact List
Ming Zhang |
contact affiliation | College of Life Sciences, Capital Normal University No. 105 Xisanhuan North Road, Beijing 100048 P. R. China |
contact email | zhangming5152007@163.com |
lab head | |
ming zhang |
contact affiliation | school of life sciences |
contact email | zhangming5152007@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000806
- Label: PRIDE project
- Name: wheat seed phosphoproteome under water deficit