PXD000804

PXD000804 is an original dataset announced via ProteomeXchange.

Title	Exploring potential virulence regulators in Paracoccidioides brasiliensis isolates of varying virulence through quantitative proteomics
Description	Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis, an important systemic mycosis in Latin America. Few virulence factors have been identified for this pathogen. In this study, we quantitatively evaluated the protein composition of P. brasiliensis in the yeast phase using minimal and rich media to obtain a better understanding of its virulence and to gain new insights into pathogen adaptation strategies. This analysis was performed on two isolates of the Pb18 strain showing distinct infection profiles in B10.A mice. Using liquid chromatography/tandem mass spectrometry (LC-MS/MS) analysis, we identified and quantified 317 proteins in minimal medium, 29 of which were overexpressed in virulent Pb18. In rich medium, 29 out of 214 identified proteins were over-expressed in the virulent fungus. Alcohol dehydrogenase, mitochondrial peroxiredoxin PRX1 and protein vacuolar ATP synthase catalytic subunit A were up-regulated in virulent Pb18 in both media, suggesting potential roles of these proteins in virulence regulation in P. brasiliensis. Proteins up-regulated in both isolates were grouped according to their functional categories. Virulent Pb18 undergoes metabolic reorganization and increased expression of proteins involved in fermentative respiration. This approach allowed us to identify potential virulence regulators and to understand how Paracoccidioides modulates the host-pathogen interaction to its advantage. Data are available via ProteomeXchange Consortium with identifier PXD000804.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_03:08:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexandre Keiji Tashima
SpeciesList	scientific name: Paracoccidioides brasiliensis (strain Pb18); NCBI TaxID: 502780;
ModificationList	deamidated residue; monohydroxylated residue; iodoacetamide derivatized residue; 2x(13)C: 4x(2)H labeled dimethylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-03-07 05:14:49	ID requested
1	2014-08-28 04:42:22	announced
2	2014-09-03 02:42:11	announced	Updated publication reference for PubMed record(s): 25145636.
⏵ 3	2014-10-23 03:08:42	announced	Updated project metadata.

Castilho DG, Chaves AF, Xander P, Zelanis A, Kitano ES, Serrano SM, Tashima AK, Batista WL, Exploring potential virulence regulators in Paracoccidioides brasiliensis isolates of varying virulence through quantitative proteomics. J Proteome Res, 13(10):4259-71(2014) [pubmed]

curator keyword: Biomedical

submitter keyword: Paracoccidioides brasiliensis

pathogenic fungus

proteomic analysis

virulence regulators

Alexandre Keiji Tashima
contact affiliation	Departamento de Bioquimica, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, SP, Brazil
contact email	aktashima@unifesp.br
lab head
Alexandre Keiji Tashima
contact affiliation	Universidade Federal de Sao Paulo
contact email	aktashima@unifesp.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000804
     1. Label: PRIDE project
     2. Name: Exploring potential virulence regulators in Paracoccidioides brasiliensis isolates of varying virulence through quantitative proteomics