PXD000802

PXD000802 is an original dataset announced via ProteomeXchange.

Title	Profiling the secretome and extracellular proteome of the potato late blight pathogen Phytophthora infestans
Description	Oomycetes are filamentous organisms that cause notorious diseases, several of which with high economic impact. Well known is Phytophthora infestans, the causal agent of potato late blight. Previously, in silico analyses of the genome and transcriptome of P. infestans resulted in the annotation of a large number of genes encoding proteins with an N-terminal signal peptide. This set is collectively referred to as the secretome and comprises proteins involved in, for example, cell wall growth and modification, proteolytic processes and the promotion of successful invasion of plant cells. So far, proteomic profiling in oomycetes was primarily focussed on subcellular, intracellular or cell wall fractions; the extracellular proteome has not been studied systematically. Here we present the first comprehensive characterization of the in vivo secretome and extracellular proteome of P. infestans. We have used mass spectrometry to analyse P. infestans proteins present in seven different growth media with mycelial cultures and this resulted in the consistent identification of over two hundred proteins. Gene ontology classification pinpointed proteins involved in cell wall modifications, pathogenesis, defense responses and proteolytic processes. Moreover, we found members of the RXLR and CRN effector families as well as several proteins lacking an obvious signal peptide. The latter were confirmed to be bona fide extracellular proteins and this suggests that, similar to other organisms, oomycetes exploit non-conventional secretion mechanisms to transfer certain proteins to the extracellular environment.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:19:47.067.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francesco Mattia Mancuso
SpeciesList	scientific name: Phytophthora infestans; NCBI TaxID: 4787;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-03-06 01:36:48	ID requested
1	2016-07-08 19:20:01	announced
⏵ 2	2024-10-22 04:19:50	announced	2024-10-22: Updated project metadata.

Meijer HJ, Mancuso FM, Espadas G, Seidl MF, Chiva C, Govers F, Sabid, ó E, Profiling the secretome and extracellular proteome of the potato late blight pathogen Phytophthora infestans. Mol Cell Proteomics, 13(8):2101-13(2014) [pubmed]

10.1074/mcp.M113.035873;

ProteomeXchange project tag: PRIME-XS Project

submitter keyword: virulence, oomycete, extracellular proteome,Secretome, Phytophthora

Eduard Sabidò
contact affiliation	UPF/CRG Proteomics Unit
contact email	eduard.sabido@crg.eu
lab head
Francesco Mattia Mancuso
contact affiliation	CRG/UPF Proteomics Unit
contact email	francescomattia.mancuso@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD000802

PRIDE project URI

• PRIDE
  1. PXD000802
     1. Label: PRIDE project
     2. Name: Profiling the secretome and extracellular proteome of the potato late blight pathogen Phytophthora infestans