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PXD000802

PXD000802 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProfiling the secretome and extracellular proteome of the potato late blight pathogen Phytophthora infestans
DescriptionOomycetes are filamentous organisms that cause notorious diseases, several of which with high economic impact. Well known is Phytophthora infestans, the causal agent of potato late blight. Previously, in silico analyses of the genome and transcriptome of P. infestans resulted in the annotation of a large number of genes encoding proteins with an N-terminal signal peptide. This set is collectively referred to as the secretome and comprises proteins involved in, for example, cell wall growth and modification, proteolytic processes and the promotion of successful invasion of plant cells. So far, proteomic profiling in oomycetes was primarily focussed on subcellular, intracellular or cell wall fractions; the extracellular proteome has not been studied systematically. Here we present the first comprehensive characterization of the in vivo secretome and extracellular proteome of P. infestans. We have used mass spectrometry to analyse P. infestans proteins present in seven different growth media with mycelial cultures and this resulted in the consistent identification of over two hundred proteins. Gene ontology classification pinpointed proteins involved in cell wall modifications, pathogenesis, defense responses and proteolytic processes. Moreover, we found members of the RXLR and CRN effector families as well as several proteins lacking an obvious signal peptide. The latter were confirmed to be bona fide extracellular proteins and this suggests that, similar to other organisms, oomycetes exploit non-conventional secretion mechanisms to transfer certain proteins to the extracellular environment.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:19:47.067.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrancesco Mattia Mancuso
SpeciesList scientific name: Phytophthora infestans; NCBI TaxID: 4787;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-03-06 01:36:48ID requested
12016-07-08 19:20:01announced
22024-10-22 04:19:50announced2024-10-22: Updated project metadata.
Publication List
Meijer HJ, Mancuso FM, Espadas G, Seidl MF, Chiva C, Govers F, Sabid, ó E, Profiling the secretome and extracellular proteome of the potato late blight pathogen Phytophthora infestans. Mol Cell Proteomics, 13(8):2101-13(2014) [pubmed]
10.1074/mcp.M113.035873;
Keyword List
ProteomeXchange project tag: PRIME-XS Project
submitter keyword: virulence, oomycete, extracellular proteome,Secretome, Phytophthora
Contact List
Eduard Sabidò
contact affiliationUPF/CRG Proteomics Unit
contact emaileduard.sabido@crg.eu
lab head
Francesco Mattia Mancuso
contact affiliationCRG/UPF Proteomics Unit
contact emailfrancescomattia.mancuso@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
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