PXD000802 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Profiling the secretome and extracellular proteome of the potato late blight pathogen Phytophthora infestans |
Description | Oomycetes are filamentous organisms that cause notorious diseases, several of which with high economic impact. Well known is Phytophthora infestans, the causal agent of potato late blight. Previously, in silico analyses of the genome and transcriptome of P. infestans resulted in the annotation of a large number of genes encoding proteins with an N-terminal signal peptide. This set is collectively referred to as the secretome and comprises proteins involved in, for example, cell wall growth and modification, proteolytic processes and the promotion of successful invasion of plant cells. So far, proteomic profiling in oomycetes was primarily focussed on subcellular, intracellular or cell wall fractions; the extracellular proteome has not been studied systematically. Here we present the first comprehensive characterization of the in vivo secretome and extracellular proteome of P. infestans. We have used mass spectrometry to analyse P. infestans proteins present in seven different growth media with mycelial cultures and this resulted in the consistent identification of over two hundred proteins. Gene ontology classification pinpointed proteins involved in cell wall modifications, pathogenesis, defense responses and proteolytic processes. Moreover, we found members of the RXLR and CRN effector families as well as several proteins lacking an obvious signal peptide. The latter were confirmed to be bona fide extracellular proteins and this suggests that, similar to other organisms, oomycetes exploit non-conventional secretion mechanisms to transfer certain proteins to the extracellular environment. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:19:47.067.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Francesco Mattia Mancuso |
SpeciesList | scientific name: Phytophthora infestans; NCBI TaxID: 4787; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-03-06 01:36:48 | ID requested | |
1 | 2016-07-08 19:20:01 | announced | |
⏵ 2 | 2024-10-22 04:19:50 | announced | 2024-10-22: Updated project metadata. |
Publication List
Meijer HJ, Mancuso FM, Espadas G, Seidl MF, Chiva C, Govers F, Sabid, ó E, Profiling the secretome and extracellular proteome of the potato late blight pathogen Phytophthora infestans. Mol Cell Proteomics, 13(8):2101-13(2014) [pubmed] |
10.1074/mcp.M113.035873; |
Keyword List
ProteomeXchange project tag: PRIME-XS Project |
submitter keyword: virulence, oomycete, extracellular proteome,Secretome, Phytophthora |
Contact List
Eduard Sabidò |
contact affiliation | UPF/CRG Proteomics Unit |
contact email | eduard.sabido@crg.eu |
lab head | |
Francesco Mattia Mancuso |
contact affiliation | CRG/UPF Proteomics Unit |
contact email | francescomattia.mancuso@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000802
- Label: PRIDE project
- Name: Profiling the secretome and extracellular proteome of the potato late blight pathogen Phytophthora infestans