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PXD000801

PXD000801 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEffects of Campylobacter jejuni CJIE1 prophage on protein expression experiment 4
DescriptionTemperate bacteriophages (prophages) have recently been demonstrated in Campylobacter jejuni. However, what they do there is largely unknown. In the series of studies that are the subject of these submissions we have investigated the relative expression levels of proteins in C. jejuni isolates that differ in the presence or absence of the CJIE1 prophage. At the time of the initial investigations whole genome sequence data were not available for the isolates used, though DNA microarray data indicated that the isolates were very closely related. The overall project was carried out through four separate experiments. Previous work in the scientific literature indicated that growth on medium lacking blood but containing sodium deoxycholate induced the expression of at least some proteins associated with virulence and provided data thought to be of relevance to the virulence of the bacterium. The second set of experiments (experiment 2) compared protein expression in 4-plex iTRAQ experiments using two isolates grown on medium with and without 0.1% sodium deoxycholate to further explore the effects of the CJIE1 prophage, if any, on protein expression. Results from that experiment indicated that there was an unexpectedly large bile response, and that there were differences in expression from the first experiment that may be attributable to the presence of blood in the culture medium in the first experiment, compared with the use of medium without blood in the second experiment. Experiment 3 was therefore designed to compare protein expression of the CJIE1-carrying isolate 00-2425 after growth on medium containing blood, without blood, and without blood but with bile salts. In experiment 4 we grew isolate 00-2426, which lacks prophage CJIE1, on the same four media. The goals were to determine if the differences in protein expression (detection) were similar to those in isolate 00-2425 and if there were any differences that could be attributed to the presence or absence of the CJIE1 prophage.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:20:10.451.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterClifford Clark
SpeciesList scientific name: Campylobacter jejuni subsp. jejuni; NCBI TaxID: 32022;
ModificationListiTRAQ4plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-03-06 01:16:23ID requested
12014-07-31 03:59:11announced
22014-12-02 00:31:30announcedUpdated project metadata.
32024-10-22 04:20:15announced2024-10-22: Updated project metadata.
Publication List
Clark CG, Chong PM, McCorrister SJ, Simon P, Walker M, Lee DM, Nguy K, Cheng K, Gilmour MW, Westmacott GR, The CJIE1 prophage of Campylobacter jejuni affects protein expression in growth media with and without bile salts. BMC Microbiol, 14():70(2014) [pubmed]
10.1186/1471-2180-14-70;
Keyword List
curator keyword: Biological
submitter keyword: Campylobacter jejun, iTRAQ,LC-MSMS,
CJIE1 prophage
Contact List
Garrett R. Westmacott
contact affiliationNational Microbiology Laboratory, Public Health Agency of Canada Proteomics and Mass Spectrometry Core Facility
contact emailGarrett.Westmacott@phac-aspc.gc.ca
lab head
Clifford Clark
contact affiliationNational Microbiology Laboratory, Public Health Agency of Canada
contact emailclifford.clark@phac-aspc.gc.ca
dataset submitter
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Dataset FTP location
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