PXD000800

PXD000800 is an original dataset announced via ProteomeXchange.

Title	Effects of Campylobacter jejuni CJIE1 prophage on protein expression experiment 3
Description	Temperate bacteriophages (prophages) have recently been demonstrated in Campylobacter jejuni. However, what they do there is largely unknown. In the series of studies that are the subject of these submissions we have investigated the relative expression levels of proteins in C. jejuni isolates that differ in the presence or absence of the CJIE1 prophage. At the time of the initial investigations whole genome sequence data were not available for the isolates used, though DNA microarray data indicated that the isolates were very closely related. The overall project was carried out through four separate experiments. In experiment 1, relative levels of protein expression of three isolates carryint the CJIE1 prophage were compared with one lacking the prophage after growth on Mueller-Hinton agar containing blood. Previous work in the scientific literature indicated that growth on medium lacking blood but containing sodium deoxycholate induced the expression of at least some proteins associated with virulence and provided data thought to be of relevance to the virulence of the bacterium. Therefore experiment 2 was done (previous submission) to evaluate in a single 4-plex iTRAQ experiment the effect of sodium deoxycholate on protein expression and whether the presence of the CJIE1 prophage had any effect. The third set of experiments (experiment 3) was done to consolidate the previous observations into a single experiment for a single strain. In three replicate experiments C. jejuni isolate 00-2425 was grown on Mueller Hinton (MH) agar base, MH agar + 10% blood, MH agar containing 0.1% sodium deoxycholate and, to further investigate the nature and extent of the bile response, MH agar containing 2.5% Oxgall.
HostingRepository	PRIDE
AnnounceDate	2014-12-02
AnnouncementXML	Submission_2014-12-02_00:31:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clifford Clark
SpeciesList	scientific name: Campylobacter jejuni subsp. jejuni; NCBI TaxID: 32022;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-03-06 01:16:00	ID requested
1	2014-07-31 03:52:31	announced
⏵ 2	2014-12-02 00:31:25	announced	Updated project metadata.

Clark CG, Chong PM, McCorrister SJ, Simon P, Walker M, Lee DM, Nguy K, Cheng K, Gilmour MW, Westmacott GR, The CJIE1 prophage of Campylobacter jejuni affects protein expression in growth media with and without bile salts. BMC Microbiol, 14():70(2014) [pubmed]

curator keyword: Biological

submitter keyword: LC-MSMS, Campylobacter jejuni, CJIE1 prophage, iTRAQ,

Garrett R. Westmacott
contact affiliation	National Microbiology Laboratory, Public Health Agency of Canada Proteomics and Mass Spectroscopy Core Facility
contact email	Garrett.Westmacott@phac-aspc.gc.ca
lab head
Clifford Clark
contact affiliation	National Microbiology Laboratory, Public Health Agency of Canada
contact email	clifford.clark@phac-aspc.gc.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000800
     1. Label: PRIDE project
     2. Name: Effects of Campylobacter jejuni CJIE1 prophage on protein expression experiment 3