PXD000799

PXD000799 is an original dataset announced via ProteomeXchange.

Title	Effects of Campylobacter jejuni CJIE1 prophage on protein expression experiment 2
Description	Temperate bacteriophages (prophages) have recently been demonstrated in Campylobacter jejuni. However, what they do there is largely unknown. In the series of studies that are the subject of these submissions we have investigated the relative expression levels of proteins in C. jejuni isolates that differ in the presence or absence of the CJIE1 prophage. At the time of the initial investigations whole genome sequence data were not available for the isolates used, though DNA microarray data indicated that the isolates were very closely related. The overall project was carried out through four separate experiments. Previous work in the scientific literature indicated that growth on medium lacking blood but containing sodium deoxycholate induced the expression of at least some proteins associated with virulence and provided data thought to be of relevance to the virulence of the bacterium. The second set of experiments (experiment 2) therefore compared protein expression in 4-plex iTRAQ experiments using two isolates. Isolate 00-2425 carried the CJIE1 prophage while the second isolate, 00-2426, did not. Three replicate experiments were done. Each isolate was grown on Mueller Hinton agar base and Mueller Hinton agar containing 0.1% sodium deoxycholate.
HostingRepository	PRIDE
AnnounceDate	2014-12-02
AnnouncementXML	Submission_2014-12-02_00:31:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clifford Clark
SpeciesList	scientific name: Campylobacter jejuni 10186; NCBI TaxID: 1365653;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-03-06 01:15:40	ID requested
1	2014-07-31 03:45:10	announced
⏵ 2	2014-12-02 00:31:21	announced	Updated project metadata.

Clark CG, Chong PM, McCorrister SJ, Simon P, Walker M, Lee DM, Nguy K, Cheng K, Gilmour MW, Westmacott GR, The CJIE1 prophage of Campylobacter jejuni affects protein expression in growth media with and without bile salts. BMC Microbiol, 14():70(2014) [pubmed]

curator keyword: Biological

submitter keyword: LC-MSMS, Campylobacter jejuni, CJIE1 prophage, iTRAQ

Garret R. Westmacott
contact affiliation	National Microbiology Laboratory, Public Health Agency of Canada Mass Spectrometry and Proteomics Core Facility
contact email	Garrett.Westmacott@phac-aspc.gc.ca
lab head
Clifford Clark
contact affiliation	National Microbiology Laboratory, Public Health Agency of Canada
contact email	clifford.clark@phac-aspc.gc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/07/PXD000799

PRIDE project URI

• PRIDE
  1. PXD000799
     1. Label: PRIDE project
     2. Name: Effects of Campylobacter jejuni CJIE1 prophage on protein expression experiment 2